Novel cobalt-based aerogels for uric acid detection in fluids at physiological pH

• Published in: Biosensors & bioelectronics 2025-01, Vol.267, p.116850, Article 116850
• Main Authors: Ruiz-Guerrero, Carlos D., Estrada-Osorio, D.V., Gutiérrez, A., Espinosa-Lagunes, F.I., Escalona-Villalpando, R.A., Luna-Bárcenas, G., Molina, A., Arenillas, A., Arriaga, L.G., Ledesma-García, J.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - methods; Cobalt - chemistry; Cobalt aerogels; Cobalt-based nanomaterials; Electrochemical Techniques - methods; Enzymes, Immobilized - chemistry; Gels - chemistry; Humans; Hydrogen-Ion Concentration; Limit of Detection; Physiological pH; Urate Oxidase - chemistry; Uric Acid - analysis; Uric Acid - blood; Uric Acid - chemistry; Uric acid oxidation; Uricase enzyme
• ISSN: 0956-5663; 1873-4235; 1873-4235
• DOI: 10.1016/j.bios.2024.116850

A sensor for uric acid (UA) based on the urate oxidase enzyme (UOx) immobilized in novel Co-based aerogels with transition metals synthesized by the sol-gel method was developed and evaluated. The Co-based aerogels: Co, Ni-Co and Pd-Co were physicochemically characterized by XRD and HR-TEM. The surface area values of 53, 57 and 66 m2 g-1 were determined for Co, Ni-Co and Pd-Co, respectively by N2 adsorption-desorption technique. Co-based aerogels were mixed by cross-linking with UOx enzymes and electrochemically characterized in buffers at pH 7.4 and 5.6 (pH values reported for biological fluids such as blood and sweat) in the presence of different uric acid concentrations. Co-based aerogels with UOx showed improved performance as a uric acid biosensor compared to using the enzyme alone. At a pH of 7.4, a higher sensitivity of 11 μA μM−1 was obtained with Pd-Co/UOx, 1.6 times higher than with UOx. At a pH value of 5.6, the highest sensitivity is achieved with Ni-Co/UOx. Stability and selectivity tests were performed in the presence of biological interferents without significant changes in the sensor. These results indicate a pleasing synergistic activity between Co-based aerogels and the enzyme. A sensor for uric acid based on the urate oxidase enzyme immobilized in novel Co-based aerogels with transition metals synthesized by the sol-gel method was developed and evaluated. [Display omitted]