Polyvinyl chloride microplastics and drought co-exposure alter rice growth by affecting metabolomics and proteomics

• Published in: The Science of the total environment 2024-12, Vol.955, p.177002, Article 177002
• Main Authors: Khan, Kiran Yasmin, Ali, Barkat, Ghani, Hafiz Usman, Cui, Xiaoqiang, Luo, Xiaohan, Ali, Zeshan, Ahmed, Waqar, Tan, Jinglu, Lysenko, Vladimir, Guo, Ya
• Format: Article
• Language: English
• Subjects: Drought; Droughts; Metabolite; Metabolomics; Microplastics - toxicity; Oryza - drug effects; Oryza - growth & development; Oryza - metabolism; Polyvinyl Chloride; Polyvinyl chloride microplastic; Protein; Proteomics; Rice; Soil Pollutants - toxicity
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.177002

Microplastics, interacting with drought stress, have become threat to crops by altering soil environment. Currently, the effect of combined microplastic and drought stress on crop growth remain poorly understood. In this work, the mechanism of multi-stress responses was investigated under the exposure of polvinylchloride microplastic (PV) and drought (D) individually and in combination (DPV) on rice varieties Hanyou73 and Q280 through proteomics and metabolomic analysis. All treatments negatively affect chlorophyll content, antioxidant enzyme activities, rice grain composition, metabolome and proteomic profiling of both rice varieties. Full rice grain yield was decreased under all treatments except PV treatment in which it was increased in both rice varieties. DPV treatment shows the lowest grain yield and more adverse effects on metabolome by affecting glycerophospholipid metabolism, tryptophan metabolism and alanine, aspartate and glutamate metabolism. Soluble sugar contents were decreased in H73 but in Q280 increased by 159 % under DPV and 123 % in PV treatment, compared to their control group. The results from metabolomics illustrate that glycerophospholipid metabolism is commonly altered in both rice types under all treatments. PV and drought alone and in combination induce extensive alterations in proteomics of rice leaves especially impacting proteins related to binding, translation and photosynthetic process. The results reveal that PV and DPV treatments highly distort the abundance of metabolites and proteins in both rice types, demonstrating that microplastic toxicity effects on rice plants become more severe when combined with drought stress. [Display omitted] •PVC treatment increased the full rice grain yield.•Drought and PVC co-exposure decreased rice grain yield.•Drought and PVC co-exposure adversely affect metabolite and protein accumulation.•CAT activity decreased in H73 but enhanced in Q280 rice leaves.