Histological and photoacoustic evaluation of rectal cancer after neoadjuvant therapy using microvascular density

Aim As non‐operative management of rectal cancer proliferates, re‐staging and surveillance methods are critical in selecting appropriate patients for organ preservation versus proctectomy. In previous work, the authors have shown that transrectal acoustic resolution photoacoustic microscopy (ARPAM)...

Full description

Saved in:
Bibliographic Details
Published in:Colorectal disease 2024-12, Vol.26 (12), p.2112-2120
Main Authors: Eltahir, Ahmed A., Nie, Haolin, Kou, Sitai, Marolt, Clayton, Navale, Pooja, Mutch, Matthew G., Chapman, William C., Zhu, Quing
Format: Article
Language:English
Subjects:
Adenocarcinoma
Adenocarcinoma - blood supply
Adenocarcinoma - diagnostic imaging
Adenocarcinoma - pathology
Adenocarcinoma - therapy
Adult
Aged
Cancer therapies
Colorectal cancer
Disease management
Endothelium
ERG
Female
Humans
Immunohistochemistry
Male
Microscopy
Microvascular Density
Microvasculature
Middle Aged
Neoadjuvant Therapy - methods
Nuclei
photoacoustic
Photoacoustic Techniques - methods
Platelet Endothelial Cell Adhesion Molecule-1 - analysis
Platelet Endothelial Cell Adhesion Molecule-1 - metabolism
Proctectomy - methods
rectal cancer
Rectal Neoplasms - blood supply
Rectal Neoplasms - diagnostic imaging
Rectal Neoplasms - pathology
Rectal Neoplasms - therapy
Rectum
Transcriptional Regulator ERG
Tumors
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aim As non‐operative management of rectal cancer proliferates, re‐staging and surveillance methods are critical in selecting appropriate patients for organ preservation versus proctectomy. In previous work, the authors have shown that transrectal acoustic resolution photoacoustic microscopy (ARPAM) co‐registered with ultrasound can differentiate residual cancer from complete tumoural response to neoadjuvant therapy. We hypothesize that these findings are due to changes in microvascular density (MVD). Methods Patients with rectal adenocarcinoma who underwent neoadjuvant therapy, transrectal photoacoustic imaging and resection were included. We first compared immunohistochemical staining with erythroblast transformation‐specific‐related gene (ERG) immunostain to standard CD31 to confirm adequate identification of endothelium. Tissue sections from identical blocks were stained with CD31 and ERG, and then a correlation was calculated between manually labelled CD31‐stained vessels and ERG nuclei density. Second, representative tissue blocks from responders, partial responders and non‐responders were stained with ERG. ERG nuclei density was quantified as a proxy for MVD. Results CD31 MVD and ERG nuclei density were strongly correlated (R2 = 0.87; P 
ISSN:1462-8910
1463-1318
1463-1318
DOI:10.1111/codi.17204