Comparative pathogenicity of duck hepatitis A virus genotype 3 in different duck breeds: Implications of the diagnosis and prevention of duck viral hepatitis

Duck hepatitis A virus (DHAV) infection in ducklings causes acute hepatitis with considerable economic losses. In this study, Pekin and Muscovy duckling flocks (n=9) suffering from high mortality and hepatic lesions were examined by RT-PCR for DVHA. 44.4 % (4/9) of samples were positive for DHAV (5′...

Full description

Saved in:
Bibliographic Details
Published in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2024-11, Vol.114, p.102256, Article 102256
Main Authors: Shawki, Mohamed M., Abido, Ola Y., Saif, Mohamed A., Sobh, Mohammed S., Gado, Ahmed R., Elnaggar, Arwa, Nassif, Samir A., El-Shall, Nahed A.
Format: Article
Language:English
Subjects:
Animals
Capsid Proteins - genetics
DHAV
Duck hepatitis virus
Ducks - virology
DVH
Genotype
Hepatitis Virus, Duck - genetics
Hepatitis Virus, Duck - pathogenicity
Hepatitis, Viral, Animal - virology
Immunity
Liver - pathology
Liver - virology
Muscovy
Pathogenicity
PCR
Pekin
Phylogeny
Picornaviridae Infections - veterinary
Picornaviridae Infections - virology
Poultry Diseases - virology
Prevention
Susceptibility
Viral Structural Proteins
Virulence
Virus Shedding
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Duck hepatitis A virus (DHAV) infection in ducklings causes acute hepatitis with considerable economic losses. In this study, Pekin and Muscovy duckling flocks (n=9) suffering from high mortality and hepatic lesions were examined by RT-PCR for DVHA. 44.4 % (4/9) of samples were positive for DHAV (5′ UTR region), of which 100 % (4/4) were DVHA-3 (VP1 gene). VP1 sequencing and phylogenetic analysis of an isolate originated from Muscovy ducklings showed that it shared 96.8 % −100 %, 88.5–89.2 %, and 86.5–88.2 % nucleotide similarity (ns) with the Egyptian, Korean-Vietnamese, and Chinese DVHA-3 strains, respectively. It was distinguished from the DHAV-1 vaccine (67.6 % ns). The sequenced DVHA-3 isolate was used to experimentally infect 5-day-old Pekin and Muscovy ducklings vs. control groups. No apparent clinical signs or deaths were reported in the experimentally-infected groups. Pekin ducklings showed greater cloacal viral shedding than Muscovy until the 6th dpi (P
ISSN:0147-9571
1878-1667
1878-1667
DOI:10.1016/j.cimid.2024.102256