Ling-Gui-Zhu-Gan decoction inhibits cardiomyocyte pyroptosis via the NLRP3/Caspase-1 signaling pathway

• Published in: Tissue & cell 2024-12, Vol.91, p.102588, Article 102588
• Main Authors: Zhao, Xiao-ni, Ding, Hui-min, Ma, Yao-yao, Wang, Liang, Zhou, Peng
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; Animals; Caspase 1 - metabolism; Cell Line; Drugs, Chinese Herbal - pharmacology; Interleukin-1beta - metabolism; Ling-Gui-Zhu-Gan decoction; Lipopolysaccharides - pharmacology; Myocardial protection; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; NLRP3/Caspase-1 pathway; Pyroptosis - drug effects; Rats; Signal Transduction - drug effects
• ISSN: 0040-8166; 1532-3072; 1532-3072
• DOI: 10.1016/j.tice.2024.102588

The objective of this study was to investigate the protective mechanism of Ling-Gui-Zhu-Gan decoction (LGZGD) against LPS-ATP-induced pyroptosis in H9c2 cells. LPS and ATP were used to induce pyroptosis in the H9c2 cell, and the cells were divided into the control, model and LGZGD groups. LDH level was detected using a colorimetric assay. ELISA was used to detect the expressions of IL-1β. Flow cytometry was utilized to observe apoptosis, while Hoechst/PI staining was used to detect pyroptosis. Immunofluorescence was employed to observe the expression levels of NLRP3 in cardiomyocytes, and RT-PCR was used to detect NLRP3, Caspase-1, GSDMD, and ASC mRNA expression. The cells were separated into seven groups: control, model, LGZGD, MCC950, LGZGD+MCC950, Nigericin and LGZGD+Nigericin. The mRNA and protein expressions were determined by RT-PCR and Western blot. LPS (10 μg/mL) for 12 h and ATP (8 mM) for 2 h were used as modeling condition. LGZGD demonstrated a significant reduction in LDH, and IL-1β levels (P