Antibacterial effects and mechanisms of quercetin-β-cyclodextrin complex mediated photodynamic on Escherichia coli O157:H7

• Published in: Archives of microbiology 2024-11, Vol.206 (11), p.445, Article 445
• Main Authors: Liu, Tao, Zhu, Yuzhang, Wang, Jiahui, Hong, Xiangyu, Liu, Mi, Kong, Chaonan, Zhou, Rui, Li, Xianke, Yang, Lifang
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antimicrobial activity; Antimicrobial agents; Bacteriostats; beta-Cyclodextrins - pharmacology; Biochemistry; Biofilms; Biofilms - drug effects; Biomedical and Life Sciences; Biotechnology; Cell Biology; Cell membranes; Cyclodextrins; Damage; E coli; Ecology; Escherichia coli; Escherichia coli O157 - drug effects; Escherichia coli O157 - radiation effects; Flavonoids; Food preservation; Fourier transforms; Gene expression; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Impact analysis; Infrared analysis; Infrared spectroscopy; Irradiation; Life Sciences; LuxS protein; Microbial Ecology; Microbial Sensitivity Tests; Microbiology; Microcapsules; MotA gene; Original Paper; Photosensitizing Agents - chemistry; Photosensitizing Agents - pharmacology; Polysaccharides; Quercetin; Quercetin - pharmacology; Spectroscopy, Fourier Transform Infrared; Thermogravimetric analysis; Ultraviolet radiation; Ultraviolet Rays; X-ray diffraction; β-Cyclodextrin
• ISSN: 0302-8933; 1432-072X; 1432-072X
• DOI: 10.1007/s00203-024-04175-1

Quercetin is a natural flavonoid with antioxidant, anti-inflammatory, and antibacterial properties. This work aimed to formulate quercetin-cyclodextrin microcapsules (QT-β-CD) while examining their photodynamic antibacterial effects and underlying mechanisms in detail. Characterization of the QT-β-CD was conducted using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The bacteriostatic effects of UV-A irradiation on Escherichia coli O157:H7 ( E. coli O157:H7) were investigated. The photodynamic impact of QT-β-CD was assessed by analyzing hydrogen peroxide (H₂O₂) production. The antimicrobial activity was further elucidated through examinations of cell membrane integrity, protein damage, changes in cellular motility, biofilm formation, and extracellular polysaccharide reduction. The effect of QT-β-CD on LuxS and motA gene expression in E. coli O157:H7 was investigated by RT-qPCR. The findings demonstrated that QT-β-CD exhibited potent photodynamic properties and functioned as an efficient photosensitizer, causing substantial damage to E. coli O157:H7 cells. These results underscore the potential of quercetin as an antimicrobial agent for food preservation.