Na+-driven pH regulation by Na+/H+ antiporters promotes photosynthetic efficiency in cyanobacteria

• Published in: Plant physiology (Bethesda) 2024-10
• Main Authors: Tsujii, Masaru, Kobayashi, Ayumu, Kano, Ayaka, Kera, Kota, Takagi, Tomoko, Nagata, Noriko, Kojima, Seiji, Hikosaka, Kouki, Oguchi, Riichi, Sonoike, Kintake, Azai, Chihiro, Inagaki, Tomomi, Ishimaru, Yasuhiro, Uozumi, Nobuyuki
• Format: Article
• Language: English
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1093/plphys/kiae562

Photosynthetic organisms have developed mechanisms to regulate light reactions in response to varying light conditions. Photosynthetic electron transport leads to the formation of a ΔpH across the thylakoid membrane, which is crucial for regulating electron transport. However, other pH modulators remain to be identified, particularly in cyanobacteria. In this study, we evaluated the potential involvement of six Na+/H+ antiporters (NhaS1-NhaS6) in control of pH in the cyanobacterium Synechocystis sp. PCC 6803. Synechocystis showed a strong requirement for Na+ at high light intensities, with ΔnhaS1 and ΔnhaS2 strains unable to grow under high light conditions. We analyzed Na+ efflux-driven H+-uptake activities of NhaS1-NhaS6 in inverted membranes of Escherichia coli. Biological fractionation and immunoelectron microscopy revealed that NhaS1 localizes to both the plasma and thylakoid membranes while NhaS2 localizes to the plasma membrane. Measurement of photosynthesis activity indicated that NhaS2 promotes ATP production and electron transport from PQ to P700. Measurements of pH outside of the cells and in the cytoplasm suggested that both NhaS1 and NhaS2 are involved in plasma membrane-mediated light-dependent H+ uptake and cytoplasmic acidification. NhaS1 and NhaS2 were also found to prevent photoinhibition under high light treatment. These results indicate that H+ transport mediated by NhaS1 and NhaS2 plays a role in regulating intracellular pH and maintaining photosynthetic electron transport.