Anti-inflammatory properties of Bacillus pumilus TS1 in lipopolysaccharide-induced inflammatory damage in broilers

• Published in: Animal biotechnology 2024-12, Vol.35 (1), p.2418516
• Main Authors: Liu, Yinkun, Liu, Sirui, Wan, Shuangshuang, Li, Zixin, Li, Hao, Tang, Shu
• Format: Article
• Language: English
• Subjects: Animal Feed - analysis; Animals; Anti-inflammatory agents; Anti-Inflammatory Agents - pharmacology; Bacillus pumilus; Bacillus pumilus - drug effects; Body weight; Chickens; Cytokines - genetics; Cytokines - metabolism; Damage detection; Feasibility studies; Flora; Gastrointestinal Microbiome - drug effects; Hsp60 protein; Hsp70 protein; HSPs; Inflammation; Inflammation - chemically induced; Inflammation - drug therapy; inflammatory; Inflammatory response; Intestinal microflora; Lipopolysaccharides; MAP kinase; NF-κB protein; Organs; P38 MAPK; Poultry Diseases - chemically induced; Poultry Diseases - drug therapy; Probiotics; Probiotics - pharmacology; Real time; Western blotting
• ISSN: 1049-5398; 1532-2378; 1532-2378
• DOI: 10.1080/10495398.2024.2418516

This study investigates whether Bacillus pumilus TS1 improves growth performance and alleviates inflammatory damage in broilers and explored its feasibility as an antibiotic alternative. We divided 240 one-day-old AA308 white-finned broilers into five groups (con, LPS, TS1L + LPS, TS1M + LPS and TS1H + LPS). The TS1L + LPS, TS1M + LPS and TS1H + LPS groups were fed TS1 for 15 days by gavage. The LPS, TS1L + LPS, TS1M + LPS and TS1H + LPS groups were injected intraperitoneally with 1 mg/kg LPS for three days. We investigated the probiotic and anti-inflammatory activities by measuring body weight, sequencing the intestinal flora and examining the structure of tissues by using pathological stain, real-time PCR, Western blotting and immunohistochemical detection. TS1 could improve growth performance and intestinal flora composition, also reduced different organ damage and inflammatory cytokine expression in serum and organs. The mechanism may involve upregulating HSP60 and HSP70 expression, targeting and regulating Nrf2 and P38 MAPK and modulating NF-κB and HO-1 expression at the transcriptional level in different organs. B. pumilus TS1 alleviated Inflammatory injury caused by LPS and attenuated the inflammatory response in broilers, and these effects were achieved through MAPK and Nrf2 regulation of HSPs/HO-1 in different organs. The above results suggested broilers fed with TS1 could release the LPS caused organ damage, and the most suggested dosage was 1.4 × 10 8  CFU/mL.