Persistent chemiluminescence-based nanosensor for portable point-of-care testing of H2O2

• Published in: Mikrochimica acta (1966) 2024-11, Vol.191 (11), p.703, Article 703
• Main Authors: Wang, Yueliang, Chen, Xiying, Tao, Zhanhui, Zhou, Jingya, Sun, Hanyue, Li, Xian, Shi, Liyu, Liu, Yaqing
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Brief Report; Characterization and Evaluation of Materials; Chemiluminescence; Chemistry; Chemistry and Materials Science; Copper selenides; Food; Hydrogen peroxide; Microengineering; Nanochemistry; Nanosensors; Nanotechnology; Peroxidase; Portable equipment; Thiols
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-024-06794-3

A novel glow-type chemiluminescence (CL)-based nanosensing system was developed for sensitive and rapid point-of-care testing (POCT) of H 2 O 2 in food. CuSe nanoparticles (CuSe NPs ) have excellent peroxidase-like activity. After being modified with thiols of 4-mercaptophenylboronic acid (MBA) (CuSe NPs @MBA), luminol can be catalyzed to produce long-lasting CL in the presence of H 2 O 2 . The possible reason for the long-lasting glow-type CL behavior was explored. Under the optimized condition, H 2 O 2 can be sensitively detected with improved repeatability. The limit of detection is as low as 0.30 μM. To meet the requirement of in situ and outside of laboratory detection, a 3D-printed portable device was designed which can eliminate the environmental interference to improve detection accuracy. The developed multifunctional platform also has the advantages of simple operation and low cost, suggesting its great potential for applications in food and agricultural fields. Graphical Abstract