Loading…
Ovariectomy drives increase of an ECM transcription signature in the posterior eye and retina
Increased risk of developing glaucoma has recently been associated with early age of menopause. Here, we examined how age and surgically-induced menopause via ovariectomy (OVX) impacted gene expression in gene pathways previously linked to glaucoma, such as extracellular matrix (ECM) remodeling and...
Saved in:
Published in: | Vision research (Oxford) 2024-12, Vol.225, p.108507, Article 108507 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | |
---|---|
cites | cdi_FETCH-LOGICAL-c241t-92a3dbacee9d53d058fd0312d3e85414c90e7fb0ed46458f32f50ed6472bace23 |
container_end_page | |
container_issue | |
container_start_page | 108507 |
container_title | Vision research (Oxford) |
container_volume | 225 |
creator | Wong, Cydney A. Sanchez-Rodriguez, Gabriela Ethier, C. Ross Wood, Levi B. Feola, Andrew J. |
description | Increased risk of developing glaucoma has recently been associated with early age of menopause. Here, we examined how age and surgically-induced menopause via ovariectomy (OVX) impacted gene expression in gene pathways previously linked to glaucoma, such as extracellular matrix (ECM) remodeling and TGF-β signaling. Using bulk RNA sequencing, we analyzed changes in young (3–4 months) and middle-aged (9–10 months) Long-Evans rats. We focused on posterior pole tissues (sclera and optic nerve head) but also examined the retina to compare observed changes across different tissue regions. Our results demonstrated that aging and OVX significantly alter gene expression in the sclera and optic nerve head. Generally, OVX triggered the enrichment of immune-related processes. However, OVX in young rats also led to significant enrichment of ECM and TGF-β gene sets. At the same time, these effects were diminished in middle-aged rats, indicating an age dependency of the effects of OVX on matrix-related pathways. Notably, the transcriptional factor Fos was downregulated in the posterior eye and retina in aged and OVX animals. Fos is a major regulator of cell proliferation and survival, and its dysregulation may play an important role in aging and menopause for women. These findings underscore the important role of menopause timing in modulating molecular pathways associated with glaucoma, which is consistent with clinical studies showing that early menopause may heighten the risk of developing this condition. This study also highlights the importance of considering women’s health factors, such as menopause, in understanding and managing glaucoma risk. |
doi_str_mv | 10.1016/j.visres.2024.108507 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3122638993</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0042698924001512</els_id><sourcerecordid>3122638993</sourcerecordid><originalsourceid>FETCH-LOGICAL-c241t-92a3dbacee9d53d058fd0312d3e85414c90e7fb0ed46458f32f50ed6472bace23</originalsourceid><addsrcrecordid>eNp9kE1PAyEQhonR2Fr9B8Zw9LKVBZZdLiamqR9JTS96NITCrNK0SwXapP9emq0ePZFhnncm8yB0XZJxSUpxtxzvXAwQx5RQnr-aitQnaFg2dVNUgotTNCSE00LIRg7QRYxLQkhdUXmOBkzyWlRUDNHHfKeDA5P8eo9tcDuI2HUmgI6AfYt1h6eTV5yC7qIJbpOc73B0n51O2wAZxekL8MbHBMH5gGEPOWNxgOQ6fYnOWr2KcHV8R-j9cfo2eS5m86eXycOsMJSXqZBUM7vQBkDaillSNa0lrKSWQVPxkhtJoG4XBCwXPDcZbatcCF7TQ4qyEbrt526C_95CTGrtooHVSnfgt1HlWVSwRkqWUd6jJviY_bVqE9xah70qiTqIVUvVi1UHsaoXm2M3xw3bxRrsX-jXZAbuewDynTsHQUXjoDNgXch6lfXu_w0_esGM6Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3122638993</pqid></control><display><type>article</type><title>Ovariectomy drives increase of an ECM transcription signature in the posterior eye and retina</title><source>ScienceDirect Journals</source><creator>Wong, Cydney A. ; Sanchez-Rodriguez, Gabriela ; Ethier, C. Ross ; Wood, Levi B. ; Feola, Andrew J.</creator><creatorcontrib>Wong, Cydney A. ; Sanchez-Rodriguez, Gabriela ; Ethier, C. Ross ; Wood, Levi B. ; Feola, Andrew J.</creatorcontrib><description>Increased risk of developing glaucoma has recently been associated with early age of menopause. Here, we examined how age and surgically-induced menopause via ovariectomy (OVX) impacted gene expression in gene pathways previously linked to glaucoma, such as extracellular matrix (ECM) remodeling and TGF-β signaling. Using bulk RNA sequencing, we analyzed changes in young (3–4 months) and middle-aged (9–10 months) Long-Evans rats. We focused on posterior pole tissues (sclera and optic nerve head) but also examined the retina to compare observed changes across different tissue regions. Our results demonstrated that aging and OVX significantly alter gene expression in the sclera and optic nerve head. Generally, OVX triggered the enrichment of immune-related processes. However, OVX in young rats also led to significant enrichment of ECM and TGF-β gene sets. At the same time, these effects were diminished in middle-aged rats, indicating an age dependency of the effects of OVX on matrix-related pathways. Notably, the transcriptional factor Fos was downregulated in the posterior eye and retina in aged and OVX animals. Fos is a major regulator of cell proliferation and survival, and its dysregulation may play an important role in aging and menopause for women. These findings underscore the important role of menopause timing in modulating molecular pathways associated with glaucoma, which is consistent with clinical studies showing that early menopause may heighten the risk of developing this condition. This study also highlights the importance of considering women’s health factors, such as menopause, in understanding and managing glaucoma risk.</description><identifier>ISSN: 0042-6989</identifier><identifier>ISSN: 1878-5646</identifier><identifier>EISSN: 1878-5646</identifier><identifier>DOI: 10.1016/j.visres.2024.108507</identifier><identifier>PMID: 39476526</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Aging ; Aging - physiology ; Animals ; Extracellular Matrix - metabolism ; Female ; Gene Expression Regulation - physiology ; Glaucoma ; Glaucoma - genetics ; Glaucoma - metabolism ; Glaucoma - physiopathology ; Menopause ; Optic Disk - metabolism ; Ovariectomy ; Rats ; Rats, Long-Evans ; Retina - metabolism ; RNA sequencing</subject><ispartof>Vision research (Oxford), 2024-12, Vol.225, p.108507, Article 108507</ispartof><rights>2024</rights><rights>Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c241t-92a3dbacee9d53d058fd0312d3e85414c90e7fb0ed46458f32f50ed6472bace23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39476526$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wong, Cydney A.</creatorcontrib><creatorcontrib>Sanchez-Rodriguez, Gabriela</creatorcontrib><creatorcontrib>Ethier, C. Ross</creatorcontrib><creatorcontrib>Wood, Levi B.</creatorcontrib><creatorcontrib>Feola, Andrew J.</creatorcontrib><title>Ovariectomy drives increase of an ECM transcription signature in the posterior eye and retina</title><title>Vision research (Oxford)</title><addtitle>Vision Res</addtitle><description>Increased risk of developing glaucoma has recently been associated with early age of menopause. Here, we examined how age and surgically-induced menopause via ovariectomy (OVX) impacted gene expression in gene pathways previously linked to glaucoma, such as extracellular matrix (ECM) remodeling and TGF-β signaling. Using bulk RNA sequencing, we analyzed changes in young (3–4 months) and middle-aged (9–10 months) Long-Evans rats. We focused on posterior pole tissues (sclera and optic nerve head) but also examined the retina to compare observed changes across different tissue regions. Our results demonstrated that aging and OVX significantly alter gene expression in the sclera and optic nerve head. Generally, OVX triggered the enrichment of immune-related processes. However, OVX in young rats also led to significant enrichment of ECM and TGF-β gene sets. At the same time, these effects were diminished in middle-aged rats, indicating an age dependency of the effects of OVX on matrix-related pathways. Notably, the transcriptional factor Fos was downregulated in the posterior eye and retina in aged and OVX animals. Fos is a major regulator of cell proliferation and survival, and its dysregulation may play an important role in aging and menopause for women. These findings underscore the important role of menopause timing in modulating molecular pathways associated with glaucoma, which is consistent with clinical studies showing that early menopause may heighten the risk of developing this condition. This study also highlights the importance of considering women’s health factors, such as menopause, in understanding and managing glaucoma risk.</description><subject>Aging</subject><subject>Aging - physiology</subject><subject>Animals</subject><subject>Extracellular Matrix - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation - physiology</subject><subject>Glaucoma</subject><subject>Glaucoma - genetics</subject><subject>Glaucoma - metabolism</subject><subject>Glaucoma - physiopathology</subject><subject>Menopause</subject><subject>Optic Disk - metabolism</subject><subject>Ovariectomy</subject><subject>Rats</subject><subject>Rats, Long-Evans</subject><subject>Retina - metabolism</subject><subject>RNA sequencing</subject><issn>0042-6989</issn><issn>1878-5646</issn><issn>1878-5646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kE1PAyEQhonR2Fr9B8Zw9LKVBZZdLiamqR9JTS96NITCrNK0SwXapP9emq0ePZFhnncm8yB0XZJxSUpxtxzvXAwQx5RQnr-aitQnaFg2dVNUgotTNCSE00LIRg7QRYxLQkhdUXmOBkzyWlRUDNHHfKeDA5P8eo9tcDuI2HUmgI6AfYt1h6eTV5yC7qIJbpOc73B0n51O2wAZxekL8MbHBMH5gGEPOWNxgOQ6fYnOWr2KcHV8R-j9cfo2eS5m86eXycOsMJSXqZBUM7vQBkDaillSNa0lrKSWQVPxkhtJoG4XBCwXPDcZbatcCF7TQ4qyEbrt526C_95CTGrtooHVSnfgt1HlWVSwRkqWUd6jJviY_bVqE9xah70qiTqIVUvVi1UHsaoXm2M3xw3bxRrsX-jXZAbuewDynTsHQUXjoDNgXch6lfXu_w0_esGM6Q</recordid><startdate>202412</startdate><enddate>202412</enddate><creator>Wong, Cydney A.</creator><creator>Sanchez-Rodriguez, Gabriela</creator><creator>Ethier, C. Ross</creator><creator>Wood, Levi B.</creator><creator>Feola, Andrew J.</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202412</creationdate><title>Ovariectomy drives increase of an ECM transcription signature in the posterior eye and retina</title><author>Wong, Cydney A. ; Sanchez-Rodriguez, Gabriela ; Ethier, C. Ross ; Wood, Levi B. ; Feola, Andrew J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c241t-92a3dbacee9d53d058fd0312d3e85414c90e7fb0ed46458f32f50ed6472bace23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Aging</topic><topic>Aging - physiology</topic><topic>Animals</topic><topic>Extracellular Matrix - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation - physiology</topic><topic>Glaucoma</topic><topic>Glaucoma - genetics</topic><topic>Glaucoma - metabolism</topic><topic>Glaucoma - physiopathology</topic><topic>Menopause</topic><topic>Optic Disk - metabolism</topic><topic>Ovariectomy</topic><topic>Rats</topic><topic>Rats, Long-Evans</topic><topic>Retina - metabolism</topic><topic>RNA sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wong, Cydney A.</creatorcontrib><creatorcontrib>Sanchez-Rodriguez, Gabriela</creatorcontrib><creatorcontrib>Ethier, C. Ross</creatorcontrib><creatorcontrib>Wood, Levi B.</creatorcontrib><creatorcontrib>Feola, Andrew J.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Vision research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wong, Cydney A.</au><au>Sanchez-Rodriguez, Gabriela</au><au>Ethier, C. Ross</au><au>Wood, Levi B.</au><au>Feola, Andrew J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ovariectomy drives increase of an ECM transcription signature in the posterior eye and retina</atitle><jtitle>Vision research (Oxford)</jtitle><addtitle>Vision Res</addtitle><date>2024-12</date><risdate>2024</risdate><volume>225</volume><spage>108507</spage><pages>108507-</pages><artnum>108507</artnum><issn>0042-6989</issn><issn>1878-5646</issn><eissn>1878-5646</eissn><abstract>Increased risk of developing glaucoma has recently been associated with early age of menopause. Here, we examined how age and surgically-induced menopause via ovariectomy (OVX) impacted gene expression in gene pathways previously linked to glaucoma, such as extracellular matrix (ECM) remodeling and TGF-β signaling. Using bulk RNA sequencing, we analyzed changes in young (3–4 months) and middle-aged (9–10 months) Long-Evans rats. We focused on posterior pole tissues (sclera and optic nerve head) but also examined the retina to compare observed changes across different tissue regions. Our results demonstrated that aging and OVX significantly alter gene expression in the sclera and optic nerve head. Generally, OVX triggered the enrichment of immune-related processes. However, OVX in young rats also led to significant enrichment of ECM and TGF-β gene sets. At the same time, these effects were diminished in middle-aged rats, indicating an age dependency of the effects of OVX on matrix-related pathways. Notably, the transcriptional factor Fos was downregulated in the posterior eye and retina in aged and OVX animals. Fos is a major regulator of cell proliferation and survival, and its dysregulation may play an important role in aging and menopause for women. These findings underscore the important role of menopause timing in modulating molecular pathways associated with glaucoma, which is consistent with clinical studies showing that early menopause may heighten the risk of developing this condition. This study also highlights the importance of considering women’s health factors, such as menopause, in understanding and managing glaucoma risk.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>39476526</pmid><doi>10.1016/j.visres.2024.108507</doi><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0042-6989 |
ispartof | Vision research (Oxford), 2024-12, Vol.225, p.108507, Article 108507 |
issn | 0042-6989 1878-5646 1878-5646 |
language | eng |
recordid | cdi_proquest_miscellaneous_3122638993 |
source | ScienceDirect Journals |
subjects | Aging Aging - physiology Animals Extracellular Matrix - metabolism Female Gene Expression Regulation - physiology Glaucoma Glaucoma - genetics Glaucoma - metabolism Glaucoma - physiopathology Menopause Optic Disk - metabolism Ovariectomy Rats Rats, Long-Evans Retina - metabolism RNA sequencing |
title | Ovariectomy drives increase of an ECM transcription signature in the posterior eye and retina |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T16%3A19%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ovariectomy%20drives%20increase%20of%20an%20ECM%20transcription%20signature%20in%20the%20posterior%20eye%20and%20retina&rft.jtitle=Vision%20research%20(Oxford)&rft.au=Wong,%20Cydney%20A.&rft.date=2024-12&rft.volume=225&rft.spage=108507&rft.pages=108507-&rft.artnum=108507&rft.issn=0042-6989&rft.eissn=1878-5646&rft_id=info:doi/10.1016/j.visres.2024.108507&rft_dat=%3Cproquest_cross%3E3122638993%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c241t-92a3dbacee9d53d058fd0312d3e85414c90e7fb0ed46458f32f50ed6472bace23%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3122638993&rft_id=info:pmid/39476526&rfr_iscdi=true |