Reprogramming of epidermal keratinocytes by PITX1 transforms the cutaneous cellular landscape and promotes wound healing

• Published in: JCI insight 2024-12, Vol.9 (24)
• Main Authors: Overmiller, Andrew M, Uchiyama, Akihiko, Hope, Emma D, Nayak, Subhashree, O'Neill, Christopher G, Hasneen, Kowser, Chen, Yi-Wen, Naz, Faiza, Dell'Orso, Stefania, Brooks, Stephen R, Jiang, Kan, Morasso, Maria I
• Format: Article
• Language: English
• Subjects: Animals; Cell Differentiation - genetics; Cell Movement - genetics; Cell Proliferation - genetics; Cellular Reprogramming - genetics; Epidermis - metabolism; Keratinocytes - metabolism; Mice; Mouth Mucosa - cytology; Mouth Mucosa - metabolism; Paired Box Transcription Factors - genetics; Paired Box Transcription Factors - metabolism; Skin - cytology; Skin - metabolism; Wound Healing - genetics
• ISSN: 2379-3708; 2379-3708
• DOI: 10.1172/jci.insight.182844

Cutaneous wound healing is a slow process that often terminates with permanent scarring while oral wounds, in contrast, regenerate after damage faster. Unique molecular networks in epidermal and oral epithelial keratinocytes contribute to the tissue-specific response to wounding, but key factors that establish those networks and how the keratinocytes interact with their cellular environment remain to be elucidated. The transcription factor PITX1 is highly expressed in the oral epithelium but is undetectable in cutaneous keratinocytes. To delineate if PITX1 contributes to oral keratinocyte identity, cell-cell interactions, and the improved wound healing capabilities, we ectopically expressed PITX1 in the epidermis of murine skin. Using comparative analysis of murine skin and oral (buccal) mucosa with single-cell RNA-Seq and spatial transcriptomics, we found that PITX1 expression enhances epidermal keratinocyte migration and proliferation and alters differentiation to a quasi-oral keratinocyte state. PITX1+ keratinocytes reprogrammed intercellular communication between skin-resident cells to mirror buccal tissue while stimulating the influx of neutrophils that establish a pro-inflammatory environment. Furthermore, PITX1+ skin healed significantly faster than control skin via increased keratinocyte activation and migration and a tunable inflammatory environment. These results illustrate that PITX1 programs oral keratinocyte identity and cellular interactions while revealing critical downstream networks that promote wound closure.