Metagenomics and Untargeted Metabolomics Analysis Revealed the Probiotic and Postbiotic Derived from Lactiplantibacillus plantarum DPUL F232 Alleviate Whey Protein-Induced Food Allergy by Reshaping Gut Microbiota and Regulating Key Metabolites

Postbiotics have emerged as a promising alternative to probiotics. However, it remains unclear whether postbiotics can exert regulatory effects on intestinal flora and metabolism as probiotics. Thus, we investigated the effects of probiotic and postbiotic in rats with whey protein-induced food aller...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2024-11, Vol.72 (45), p.25436-25448
Main Authors: Feng, Lu, Guo, Zihao, Yao, Wenpu, Mu, Guangqing, Zhu, Xuemei
Format: Article
Language:English
Subjects:
Animals
Female
Food Hypersensitivity - immunology
Gastrointestinal Microbiome - drug effects
Humans
Immunoglobulin E - blood
Immunoglobulin E - immunology
Lactobacillus plantarum
Male
Metabolomics
Metagenomics
Omics Technologies Applied to Agriculture and Food
Probiotics - administration & dosage
Probiotics - pharmacology
Rats
Rats, Sprague-Dawley
Whey Proteins - metabolism
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Summary:Postbiotics have emerged as a promising alternative to probiotics. However, it remains unclear whether postbiotics can exert regulatory effects on intestinal flora and metabolism as probiotics. Thus, we investigated the effects of probiotic and postbiotic in rats with whey protein-induced food allergy, which demonstrated that postbiotic intervention effectively alleviated allergy symptoms, reduced serum immunoglobulin E (IgE) and mast cell protease-1 (mMCP-1) levels, and regulated the type helper 1 cell/2 cell (Th1/Th2) balance in both serum and spleen. Metagenomic analysis revealed that postbiotics induced more significant changes in intestinal flora. Untargeted metabolomics analysis showed that both probiotics and postbiotics significantly up-regulated various differential metabolites, which were negatively correlated with immune indices, including malvidin-3-glucoside, 3,4-dihydroxymandelic acid, nicotinamide, triterpenoids, pirbuterol, and 4-hydroxybenzoic acid. This study confirms that postbiotics can alleviate food allergies and regulate intestinal flora and metabolites, which provides a valuable reference for the use of postbiotics in mitigating allergic diseases through gut microbiota and metabolite modulation.
ISSN:0021-8561
1520-5118
1520-5118
DOI:10.1021/acs.jafc.4c08203