Do probiotics and iron supplementation have any impact on element distribution in rat kidneys? – bioimaging by laser ablation inductively coupled plasma mass spectrometry

• Published in: Talanta (Oxford) 2025-02, Vol.283, p.127112, Article 127112
• Main Authors: Frąckowiak, Julia, Komorowicz, Izabela, Sajnóg, Adam, Skrypnik, Katarzyna, Suliburska, Joanna, Hanć, Anetta
• Format: Article
• Language: English
• Subjects: Animals; Bioimaging; Dietary Supplements; Elements; Female; Iron; Iron - analysis; Iron - metabolism; Kidney; Kidney - chemistry; Kidney - diagnostic imaging; Kidney - metabolism; LA-ICP-MS; Laser Therapy; Mass Spectrometry - methods; Probiotics; Rats; Rats, Wistar; Trace Elements - analysis; Trace Elements - metabolism
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2024.127112

This study investigates the influence of multistrain probiotics and iron supplementation on the distribution and interaction of trace elements in the kidneys of Wistar rats using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) bioimaging. Forty 6-week-old female Wistar rats were divided into five groups, each fed an AIN-93 M diet with varying additions or deficiencies of iron and probiotics, which included a control, an iron-deficient diet, an iron-deficient diet with probiotics, an iron-deficient diet with iron supplementation, and an iron-deficient diet with both probiotics and iron supplementation. The obtained two-dimensional maps of the distribution of elements reveal distinct locations of Cu, Fe, Mn, and Zn in specific tissues of rat kidneys. Specifically, Cu and Fe were co-localized in the renal cortex, while Zn was mostly absent from regions where Cu and Fe accumulated. Fe supplementation alone increased Fe in the renal cortex, while probiotics enhanced this effect, suggesting a synergistic role in Fe absorption. The total content of elements in the kidneys of all groups was determined after digestion: Cu 13.3–24.7 mg kg−1, Fe 218–509 mg kg−1, Mn 0.87–1.29 mg kg−1, and Zn 28.6–40.1 mg kg−1. Competitive interactions among Cu, Fe, and Zn were observed, with probiotics modulating their concentrations and distribution, highlighting their role in trace element homeostasis. Our research provides insights into the interactions between dietary supplements, probiotics, and trace element distribution in kidneys, paving the way for targeted nutritional interventions. This study highlights the need for further research on trace element functions in organisms and their impact on health. [Display omitted] •Bioimaging of rat kidney by LA-ICP-MS.•Elements interaction in rat kidneys.•Multi-strain probiotics impact on trace elements distribution in rat kidneys.•Probiotics, by affecting Fe, may change the content and distribution of Cu, Zn, Mn.