miR-2110 Affects the Biological Behaviors of Lung Adenocarcinoma by Regulating CDT1

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2024-09, Vol.55 (5), p.1202
• Main Authors: Mi, Yuan, Li, Xuzhe, Wang, Cong, Lin, Chenxi, Zhao, Zhichao, Yan, Xiaofei, Shi, Ping, Wang, Lei
• Format: Article
• Language: Chinese
• Subjects: Adenocarcinoma of Lung - genetics; Adenocarcinoma of Lung - metabolism; Adenocarcinoma of Lung - pathology; Animals; Apoptosis - genetics; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation; Female; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Mice; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - genetics; MicroRNAs - metabolism; Neoplasm Metastasis - genetics
• ISSN: 1672-173X
• DOI: 10.12182/20240960505

To investigate the effect of miR-2110 on the biological behaviors, such as cell proliferation, apoptosis, and metastasis, of lung adenocarcinoma (LUAD) cells by means of cell and animal experiments. Bioinformatics websites, including ENCORI, TargetScan, miRTarBase, and Tarbase, were used to analyze the changes in the expression of miR-2110 in LUAD samples and to predict miR-2110 target. LUAD tissue samples and cells were collected and the changes in the expression of miR-2110 were verified through PCR technology. CCK-8 assay, clonogenic assay, Transwell assay, and flow cytometry were conducted to analyze alterations in the functions of LUAD cells. In addition, 10 BALB/c female nude mice aged 6 to 8 weeks were randomly divided into 2 groups, and the effect of miR-2110 on LUAD was investigated by experiments. miR-2110 was significantly decreased in LUAD tissues and cells compared with the normal lung tissues. miR-2110 overexpression inhibited the proliferation and metastasis of LUAD cells and promoted the apopt