Protective Effect and Mechanism of miR-328-3p on Coronary Artery Endothelial Cell Injury Induced by Oxidized Low-density Lipoprotein

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2024-09, Vol.55 (5), p.1210
• Main Authors: Hou, Yonglan, Li, Xia, Wang, Jianmei, Liu, Zhen, Han, Minglei, Liu, Zhenghao, Jin, Weidong
• Format: Article
• Language: Chinese
• Subjects: Apoptosis - drug effects; bcl-2-Associated X Protein - metabolism; Caspase 3; Cells, Cultured; Coronary Vessels - cytology; Endothelial Cells - metabolism; Humans; Insulin-Like Growth Factor II - genetics; Insulin-Like Growth Factor II - metabolism; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Lipoproteins, LDL; MicroRNAs - genetics; MicroRNAs - metabolism; Proto-Oncogene Proteins c-bcl-2 - genetics; Proto-Oncogene Proteins c-bcl-2 - metabolism; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1672-173X
• DOI: 10.12182/20240960601

To investigate the protective effect of miR-328-3p on oxidized low-density lipoprotein (ox-LDL)-induced coronary artery endothelial cell injury and the potentially relevant mechanisms. Human coronary artery endothelial cells (HCAECs) were induced with ox-LDL, and the cells were divided into a control group consisting of normal cells, an ox-LDL group receiving ox-LDL treatment, an ox-LDL+miR-NC group transfected with miR-NC and treated with ox-LDL, an ox-LDL+miR-328-3p group transfected with miR-328-3p and treated with ox-LDL, and ox-LDL+miR-328-3p+pcDNA group co-transfected miR-328-3p and pcDNA and treated with ox-LDL, and an ox-LDL+miR-328-3p+insulin-like growth factor 2 (IGF2) group co-transfected miR-328-3p and IGF2 and treated with ox-LDL. The expression level of miR-328-3p was determined with RT-qPCR. Cell proliferation was determined by MTT. Cell apoptosis was measured by flow cytometry. Western blot was conducted to examine the protein expression levels of cleaved cas-3 and IGF2. ELISA was performed to