Tenebrio molitor (Coleoptera: Tenebrionidae) as an alternative host for the study of pathogenicity in Candida auris

• Published in: Microbial pathogenesis 2025-01, Vol.198, p.107115, Article 107115
• Main Authors: Rodrigues, Milena Izabel dos Santos, Cruz, Gabriel Henrique Rodrigues da, Lucini, Fabíola, Almeida, Alexandre Moreira de, Pereira, Fabricio Fagundes, Ramalho, Rondon Tosta, Simionatto, Simone, Rossato, Luana
• Format: Article
• Language: English
• Subjects: Animals; Biofilms - growth & development; Candida auris - genetics; Candida auris - pathogenicity; Candidiasis - microbiology; Disease Models, Animal; Humoral response; Insect model; Larva - microbiology; Mealworm; Monophenol Monooxygenase - metabolism; Tenebrio - microbiology; Virulence
• ISSN: 0882-4010; 1096-1208; 1096-1208
• DOI: 10.1016/j.micpath.2024.107115

Candida auris, a multidrug-resistant fungal pathogen, has emerged as a significant global health threat due to its high transmission and mortality rates, especially in healthcare settings. This study aimed to establish the larvae of the coleopteran Tenebrio molitor (mealworm) as an in vivo model to evaluate the virulence of different C. auris strains. T. molitor larvae were inoculated with varying doses and strains of C. auris. Mortality rates were monitored, melanization responses, and phenoloxidase activity were assessed. Histopathological analyses were conducted to observe tissue invasion by the yeast cells. Additionally, a biofilm formation test was included as a complementary analysis to determine if biofilm production would influence the virulence of the C. auris strains. A dose-dependent increase in mortality was observed, with the highest fungal load leading to the highest mortality rates. The study also revealed significant differences in virulence among the strains, with those from Kuwait and the reference strain CBS 10913 showing the highest pathogenicity. Melanization rates were significantly higher in infected larvae, indicating an active immune response. The histopathological analysis revealed the presence of C. auris cells within the tissue of T. molitor larvae. However, the biofilm formation complementary test did not show a significant difference in virulence among the different clades of C. auris. The T. molitor model effectively demonstrated the pathogenic potential and virulence differences of C. auris strains. Strains from Kuwait and the reference strain CBS 10913 exhibited the highest virulence, causing 100 % mortality within 24 h. The model also highlighted significant biofilm formation and melanization responses, correlating with fungal burden. This insect model provides a valuable and cost-effective tool for preliminary virulence screening of clinical yeast strains, offering insights into host-pathogen interactions and the potential for evaluating antifungal treatments in vivo. •Candida auris has shown a concerning escalation in incidence, leading to severe healthcare-associated infections with high mortality rates.•Candida auris sets itself apart from other Candida species with its heightened resistance to antifungal agents and common disinfectants, along with its exceptional ability to persist in healthcare environments and survive on surfaces for extended durations.•Virulence factors include, biofilm formation and enhanced stres