On-line correlative imaging of cryo-PALM and soft X-ray tomography for identification of subcellular structures

Correlative imaging of fluorescence microscopy and soft X-ray microscopy plays a crucial role in exploring the relationship between structure and function in cellular biology. However, the current correlative imaging methods are limited either to off-line or low-resolution fluorescence imaging. In t...

Full description

Saved in:
Bibliographic Details
Published in:Optics express 2024-07, Vol.32 (16), p.27508
Main Authors: Zhang, Chao, Guan, Yong, Tao, Xiayu, Tian, Lijiao, Chen, Liang, Xiong, Ying, Liu, Gang, Wu, Zhao, Tian, Yangchao
Format: Article
Language:English
Subjects:
Humans
Microscopy, Fluorescence - methods
Tomography, X-Ray - methods
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Correlative imaging of fluorescence microscopy and soft X-ray microscopy plays a crucial role in exploring the relationship between structure and function in cellular biology. However, the current correlative imaging methods are limited either to off-line or low-resolution fluorescence imaging. In this study, we developed an integrated on-line cryogenic photoactivated localization microscopy (cryo-PALM) system at a soft X-ray microscopy station. This design eliminates some critical issues such as sample damage and complex post-correlation arising from transferring samples between different cryostages. Furthermore, we successfully achieved correlative imaging of cryopreserved near-native cells, with a resolution of about 50 nm of cryo-PALM. Therefore, the developed on-line correlation imaging platform provides a powerful tool for investigating the intricate relationship between structure and function in biological and molecular interactions, as well as in other life science disciplines.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.532138