Construction of bilayer biomimetic periosteum based on SLA-3D printing for bone regeneration

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2025-02, Vol.246, p.114368, Article 114368
• Main Authors: Zhou, Xingguo, Zou, Bin, Chen, Qinghua, Yang, Gongxian, Lai, Qingguo, Wang, Xinfeng
• Format: Article
• Language: English
• Subjects: 3D printing; Animals; Biomimetic Materials - chemistry; Biomimetic Materials - pharmacology; Biomimetic periosteum; Bone Regeneration - drug effects; Bone tissue engineering; Calcium Phosphates - chemistry; Calcium Phosphates - pharmacology; Cell Differentiation - drug effects; Chitosan - chemistry; Gelatin - chemistry; Hydrogels - chemistry; Hydrogels - pharmacology; Methacrylates - chemistry; Methacrylates - pharmacology; Mice; Molybdenum - chemistry; Molybdenum - pharmacology; Osteoblasts - cytology; Osteoblasts - drug effects; Osteogenesis - drug effects; Periosteum - cytology; Periosteum - drug effects; Photothermal Therapy; Polyethylene Glycols - chemistry; Porosity; Printing, Three-Dimensional; Surface Properties; Tissue Engineering; Tissue Scaffolds - chemistry
• ISSN: 0927-7765; 1873-4367; 1873-4367
• DOI: 10.1016/j.colsurfb.2024.114368

An ideal biomimetic periosteum should have excellent biocompatibility to promote osteoclast adhesion and improve osseointegration, which is significant in promoting bone regeneration. In this work, a bionic artificial periosteum printed by the SLA-3D printing was prepared, consisting of a poly (ethylene glycol) diacrylate (PEGDA)/chitosan/tricalcium phosphate (TCP) fibrous layer and a gelatin methacryloyl (GelMA)/ammonium molybdate (Mo) cambium layer. Distinct surface characteristics were achieved on both sides of the biomimetic periosteum. Among them, the fibrous layer has high mechanical properties and low porosity, which is conducive to preventing the pulling of muscle tissues and the invasion of soft tissues. The cambium layer has a porous structure and bioactive factors that can effectively promote osteogenic differentiation of preosteoblasts. Combined with mild photothermal therapy triggered by NIR light, the biomimetic periosteum could promote bone regeneration at both the chemical and physical levels. This 3D-printed bilayer hydrogel can provide a promising strategy for preparing advanced tissue-engineered periosteum with excellent physical and bone regeneration properties. •The bilayer biomimetic periosteum for bone regeneration was prepared using SLA-3D printing.•Both sides of the biomimetic periosteum had distinct surface characteristics.•The biomimetic periosteum can accelerate bone regeneration and promote external wound healing.