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Evaluation of intra- and inter-rater agreement on ultrasound measurements of dairy cow teats by operators with different experience levels
Ultrasound is a valuable, non-invasive technique. It allows for detailed examination, precise measurement, and effective monitoring of teats in dairy cows. This study aimed to evaluate the reliability of ultrasound measurements of teats in dairy cows, specifically focusing on intra- and inter-rater...
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Published in: | Veterinary research communications 2025-02, Vol.49 (1), p.9, Article 9 |
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description | Ultrasound is a valuable, non-invasive technique. It allows for detailed examination, precise measurement, and effective monitoring of teats in dairy cows. This study aimed to evaluate the reliability of ultrasound measurements of teats in dairy cows, specifically focusing on intra- and inter-rater agreement between operators with different levels of experience. The study included 51 healthy Holstein cows, with two operators (experienced and inexperienced) performing measurements on ultrasound images obtained before and after milking. The parameters analyzed were teat-canal length (TCL), teat-end width (TEW), teat-wall thickness (TWT), and teat-cistern width (TCW). Differences in teat measurements before and after milking, as well as between operators, were assessed using the Wilcoxon Signed Rank test and the Mann-Whitney U test, respectively. Intra- and inter-rater agreement was calculated using the intra-class correlation coefficient (ICC). Statistical significance was set at
p
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doi_str_mv | 10.1007/s11259-024-10602-x |
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p
< 0.05. A total of 402 ultrasound images were analyzed. Significant differences were found in TCL, TWT, and TCW before and after milking, while TEW remained unaffected. Statistically significant differences in TWT and TCW were observed between the two operators, while TCL and TEW did not show significant differences. Intra-rater agreement for the experienced operator was excellent, and for the inexperienced operator, it was good. Inter-rater agreement ranged from good to excellent for most parameters, except for TWT, where it was poor. The study confirms that both milking and operator experience influence teat measurements. However, with proper training, ultrasonography can be a reliable tool for assessing udder health in dairy cows, supporting its use in field conditions.</description><identifier>ISSN: 0165-7380</identifier><identifier>ISSN: 1573-7446</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-024-10602-x</identifier><identifier>PMID: 39549113</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Biomedical and Life Sciences ; Cattle ; Dairy cattle ; Dairying - methods ; Female ; Life Sciences ; Mammary Glands, Animal - diagnostic imaging ; Milking ; Observer Variation ; Reproducibility of Results ; Statistical analysis ; Teats ; Udder ; Ultrasonic imaging ; Ultrasonography - methods ; Ultrasonography - standards ; Ultrasonography - veterinary ; Ultrasound ; Veterinary Medicine/Veterinary Science ; Zoology</subject><ispartof>Veterinary research communications, 2025-02, Vol.49 (1), p.9, Article 9</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer Nature B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-31f28d253ba35bc39ea531a29d29c417f117a673e9fb05ef12ba4595526162b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39549113$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nocera, I.</creatorcontrib><creatorcontrib>Sala, G.</creatorcontrib><creatorcontrib>Orsetti, C.</creatorcontrib><creatorcontrib>Rodriguez-Lopez, J. M.</creatorcontrib><creatorcontrib>Sgorbini, M.</creatorcontrib><creatorcontrib>Bonelli, F.</creatorcontrib><title>Evaluation of intra- and inter-rater agreement on ultrasound measurements of dairy cow teats by operators with different experience levels</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>Ultrasound is a valuable, non-invasive technique. It allows for detailed examination, precise measurement, and effective monitoring of teats in dairy cows. This study aimed to evaluate the reliability of ultrasound measurements of teats in dairy cows, specifically focusing on intra- and inter-rater agreement between operators with different levels of experience. The study included 51 healthy Holstein cows, with two operators (experienced and inexperienced) performing measurements on ultrasound images obtained before and after milking. The parameters analyzed were teat-canal length (TCL), teat-end width (TEW), teat-wall thickness (TWT), and teat-cistern width (TCW). Differences in teat measurements before and after milking, as well as between operators, were assessed using the Wilcoxon Signed Rank test and the Mann-Whitney U test, respectively. Intra- and inter-rater agreement was calculated using the intra-class correlation coefficient (ICC). Statistical significance was set at
p
< 0.05. A total of 402 ultrasound images were analyzed. Significant differences were found in TCL, TWT, and TCW before and after milking, while TEW remained unaffected. Statistically significant differences in TWT and TCW were observed between the two operators, while TCL and TEW did not show significant differences. Intra-rater agreement for the experienced operator was excellent, and for the inexperienced operator, it was good. Inter-rater agreement ranged from good to excellent for most parameters, except for TWT, where it was poor. The study confirms that both milking and operator experience influence teat measurements. However, with proper training, ultrasonography can be a reliable tool for assessing udder health in dairy cows, supporting its use in field conditions.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cattle</subject><subject>Dairy cattle</subject><subject>Dairying - methods</subject><subject>Female</subject><subject>Life Sciences</subject><subject>Mammary Glands, Animal - diagnostic imaging</subject><subject>Milking</subject><subject>Observer Variation</subject><subject>Reproducibility of Results</subject><subject>Statistical analysis</subject><subject>Teats</subject><subject>Udder</subject><subject>Ultrasonic imaging</subject><subject>Ultrasonography - methods</subject><subject>Ultrasonography - standards</subject><subject>Ultrasonography - veterinary</subject><subject>Ultrasound</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Zoology</subject><issn>0165-7380</issn><issn>1573-7446</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2025</creationdate><recordtype>article</recordtype><recordid>eNp9kUFv1DAQhS0EotvCH-CALHHhYvDYsRMfUVWgUiUucLYmybikSuLFTtrdv8CvxrtbQOLAxWP5fe95pMfYK5DvQMr6fQZQxgmpKgHSSiV2T9gGTK1FXVX2KdtIsEbUupFn7DznOymla6R-zs60M5UD0Bv28-oexxWXIc48Bj7MS0LBce4PV0oiYTk53iaiieaFF2wdC5PjWpiJMK_pqOSDvcch7XkXH_hCWJ7aPY9bKhkxZf4wLN95P4RA6ZBEu6IMNHfER7qnMb9gzwKOmV4-zgv27ePV18vP4ubLp-vLDzeiU8YuQkNQTa-MblGbttOO0GhA5XrlugrqAFCjrTW50EpDAVSLlXHGKAtWtU5fsLen3G2KP1bKi5-G3NE44kxxzV6Dcgoaq6uCvvkHvYtrmst2R0oaBbYplDpRXYo5Jwp-m4YJ096D9Iem_KkpX5ryx6b8rpheP0av7UT9H8vvagqgT0Au0nxL6e_f_4n9BUWyoNI</recordid><startdate>20250201</startdate><enddate>20250201</enddate><creator>Nocera, I.</creator><creator>Sala, G.</creator><creator>Orsetti, C.</creator><creator>Rodriguez-Lopez, J. M.</creator><creator>Sgorbini, M.</creator><creator>Bonelli, F.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20250201</creationdate><title>Evaluation of intra- and inter-rater agreement on ultrasound measurements of dairy cow teats by operators with different experience levels</title><author>Nocera, I. ; Sala, G. ; Orsetti, C. ; Rodriguez-Lopez, J. M. ; Sgorbini, M. ; Bonelli, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-31f28d253ba35bc39ea531a29d29c417f117a673e9fb05ef12ba4595526162b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2025</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Cattle</topic><topic>Dairy cattle</topic><topic>Dairying - methods</topic><topic>Female</topic><topic>Life Sciences</topic><topic>Mammary Glands, Animal - diagnostic imaging</topic><topic>Milking</topic><topic>Observer Variation</topic><topic>Reproducibility of Results</topic><topic>Statistical analysis</topic><topic>Teats</topic><topic>Udder</topic><topic>Ultrasonic imaging</topic><topic>Ultrasonography - methods</topic><topic>Ultrasonography - standards</topic><topic>Ultrasonography - veterinary</topic><topic>Ultrasound</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nocera, I.</creatorcontrib><creatorcontrib>Sala, G.</creatorcontrib><creatorcontrib>Orsetti, C.</creatorcontrib><creatorcontrib>Rodriguez-Lopez, J. M.</creatorcontrib><creatorcontrib>Sgorbini, M.</creatorcontrib><creatorcontrib>Bonelli, F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nocera, I.</au><au>Sala, G.</au><au>Orsetti, C.</au><au>Rodriguez-Lopez, J. M.</au><au>Sgorbini, M.</au><au>Bonelli, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of intra- and inter-rater agreement on ultrasound measurements of dairy cow teats by operators with different experience levels</atitle><jtitle>Veterinary research communications</jtitle><stitle>Vet Res Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2025-02-01</date><risdate>2025</risdate><volume>49</volume><issue>1</issue><spage>9</spage><pages>9-</pages><artnum>9</artnum><issn>0165-7380</issn><issn>1573-7446</issn><eissn>1573-7446</eissn><abstract>Ultrasound is a valuable, non-invasive technique. It allows for detailed examination, precise measurement, and effective monitoring of teats in dairy cows. This study aimed to evaluate the reliability of ultrasound measurements of teats in dairy cows, specifically focusing on intra- and inter-rater agreement between operators with different levels of experience. The study included 51 healthy Holstein cows, with two operators (experienced and inexperienced) performing measurements on ultrasound images obtained before and after milking. The parameters analyzed were teat-canal length (TCL), teat-end width (TEW), teat-wall thickness (TWT), and teat-cistern width (TCW). Differences in teat measurements before and after milking, as well as between operators, were assessed using the Wilcoxon Signed Rank test and the Mann-Whitney U test, respectively. Intra- and inter-rater agreement was calculated using the intra-class correlation coefficient (ICC). Statistical significance was set at
p
< 0.05. A total of 402 ultrasound images were analyzed. Significant differences were found in TCL, TWT, and TCW before and after milking, while TEW remained unaffected. Statistically significant differences in TWT and TCW were observed between the two operators, while TCL and TEW did not show significant differences. Intra-rater agreement for the experienced operator was excellent, and for the inexperienced operator, it was good. Inter-rater agreement ranged from good to excellent for most parameters, except for TWT, where it was poor. The study confirms that both milking and operator experience influence teat measurements. However, with proper training, ultrasonography can be a reliable tool for assessing udder health in dairy cows, supporting its use in field conditions.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>39549113</pmid><doi>10.1007/s11259-024-10602-x</doi></addata></record> |
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subjects | Animals Biomedical and Life Sciences Cattle Dairy cattle Dairying - methods Female Life Sciences Mammary Glands, Animal - diagnostic imaging Milking Observer Variation Reproducibility of Results Statistical analysis Teats Udder Ultrasonic imaging Ultrasonography - methods Ultrasonography - standards Ultrasonography - veterinary Ultrasound Veterinary Medicine/Veterinary Science Zoology |
title | Evaluation of intra- and inter-rater agreement on ultrasound measurements of dairy cow teats by operators with different experience levels |
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