Dual activation of soybean resistance against Phytophthora sojae by pectin lyase and degraded pectin oligosaccharides

• Published in: Science China. Life sciences 2024-12, Vol.67 (12), p.2746-2760
• Main Authors: Sun, Guangzheng, Xia, Yeqiang, Li, Kuikui, Zhu, Qinsheng, Ding, Feifei, Gu, Hui, Zhang, Zhichao, Li, Xinrui, Mi, Xuan, Chen, Jun, Yao, Ruoting, Zhang, Sicong, Ouyang, Haibing, Chen, Xi, Liu, Tengfei, Jiang, Haibin, Zhao, Yao, Qiu, Min, Ye, Wenwu, Duan, Kaixuan, Ma, Zhenchuan, Dong, Suomeng, Yin, Heng, Wang, Yan, Wang, Yuanchao
• Format: Article
• Language: English
• Subjects: Acetylation; Biomedical and Life Sciences; Disease Resistance; Gene Expression Regulation, Plant; Glycine max - immunology; Glycine max - metabolism; Glycine max - microbiology; Glycine max - parasitology; Host plants; Host-pathogen interactions; Host-Pathogen Interactions - immunology; Immune response; Immunogenicity; Infections; Kinases; Life Sciences; Methylation; Oligosaccharides; Oligosaccharides - metabolism; Pathogens; Pectin; Pectin lyase; Pectins - metabolism; Phytophthora; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Diseases - parasitology; Plant Immunity; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plants, Genetically Modified; Polysaccharide-Lyases - metabolism; Polysaccharides; Receptor mechanisms; Research Paper; Soybeans; Virulence factors
• ISSN: 1674-7305; 1869-1889; 1869-1889
• DOI: 10.1007/s11427-024-2724-5

Phytophthora pathogens secrete numerous apoplastic effectors to manipulate host immunity. Herein, we identified a polysaccharide lyase 1 protein, PsPL1, which acts as an essential virulence factor of P. sojae infection in soybean. However, the overexpression of PsPL1 in P. sojae reduced infection and triggered enhanced immune responses in soybean. PsPL1 exhibited pectin lyase activity and degraded plant pectin to generate pectin oligosaccharides (POSs) with a polymerization degree of 3–14, exhibiting different levels of acetylation and methylation modifications. PsPL1 and the degraded pectin products triggered immune responses in soybean and different Solanaceous plants. The PsPL1-triggered immune responses required RSPL1, a membrane-localized leucine-rich repeat receptor-like protein, which is essential for Phytophthora resistance. Conversely, the PsPL1-degraded product-triggered immune responses depended on the membrane-localized lysin motif receptor-like kinase CERK1. This study reveals that the pectin lyase exhibits a dual immunogenic role during P. sojae infection, which activates plant resistance through different immune receptors and provides novel insights into the function of pectin lyase in host-pathogen interactions.