Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori

• Published in: Yeast (Chichester, England) England), 2024-11
• Main Authors: Kadkhodaei, Sara, Hatefi, Atousa, Pedramnia, Shahrzad, Godini, Elham, Khalili-Samani, Saman, Saniee, Parastoo, Sarrafnejad, Abdolfattah, Salmanian, Ali-Hatef, Sotoudeh, Masoud, Graham, David Y, Malekzadeh, Reza, Siavoshi, Farideh
• Format: Article
• Language: English
• ISSN: 0749-503X; 1097-0061; 1097-0061
• DOI: 10.1002/yea.3983

The relationship between oral and gastric yeasts and their role in the colonization of Helicobacter pylori in the stomach was studied. Four groups of 221, 7, 44, and 10 patients were used for the isolation of H. pylori and oral and gastric yeasts. In Group 1, gastric biopsies were used for the isolation of H. pylori and yeast, rapid urease test (RUT), staining with Gram's and hematoxylin & eosin (H&E), and immunohistochemistry (IHC) methods. In the other three groups, DNAs extracted from H. pylori and yeasts were used for the amplification of H. pylori-specific genes. Wet mounts of yeasts in Group 2 were examined to observe intracellular bacteria and released EVs. Among 221 patients, 65 (29.3%) had oral yeast, 35 (15.8%) H. pylori, and 31 (14%) gastric yeast. Culture of oral yeasts showed a significant correlation with the detection of H. pylori by IHC (10.3%), Gram stain (9%), RUT (6.3%), H&E (4.9%), and culture (4%) (p