Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori

The relationship between oral and gastric yeasts and their role in the colonization of Helicobacter pylori in the stomach was studied. Four groups of 221, 7, 44, and 10 patients were used for the isolation of H. pylori and oral and gastric yeasts. In Group 1, gastric biopsies were used for the isola...

Full description

Saved in:
Bibliographic Details
Published in:Yeast (Chichester, England) England), 2024-11
Main Authors: Kadkhodaei, Sara, Hatefi, Atousa, Pedramnia, Shahrzad, Godini, Elham, Khalili-Samani, Saman, Saniee, Parastoo, Sarrafnejad, Abdolfattah, Salmanian, Ali-Hatef, Sotoudeh, Masoud, Graham, David Y, Malekzadeh, Reza, Siavoshi, Farideh
Format: Article
Language:English
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c174t-e64189bc114e94592efb5f7f4e8761d60e2dc3eb0bdf835ec1d30676392d7fc53
container_end_page
container_issue
container_start_page
container_title Yeast (Chichester, England)
container_volume
creator Kadkhodaei, Sara
Hatefi, Atousa
Pedramnia, Shahrzad
Godini, Elham
Khalili-Samani, Saman
Saniee, Parastoo
Sarrafnejad, Abdolfattah
Salmanian, Ali-Hatef
Sotoudeh, Masoud
Graham, David Y
Malekzadeh, Reza
Siavoshi, Farideh
description The relationship between oral and gastric yeasts and their role in the colonization of Helicobacter pylori in the stomach was studied. Four groups of 221, 7, 44, and 10 patients were used for the isolation of H. pylori and oral and gastric yeasts. In Group 1, gastric biopsies were used for the isolation of H. pylori and yeast, rapid urease test (RUT), staining with Gram's and hematoxylin & eosin (H&E), and immunohistochemistry (IHC) methods. In the other three groups, DNAs extracted from H. pylori and yeasts were used for the amplification of H. pylori-specific genes. Wet mounts of yeasts in Group 2 were examined to observe intracellular bacteria and released EVs. Among 221 patients, 65 (29.3%) had oral yeast, 35 (15.8%) H. pylori, and 31 (14%) gastric yeast. Culture of oral yeasts showed a significant correlation with the detection of H. pylori by IHC (10.3%), Gram stain (9%), RUT (6.3%), H&E (4.9%), and culture (4%) (p 
doi_str_mv 10.1002/yea.3983
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3129219334</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3129219334</sourcerecordid><originalsourceid>FETCH-LOGICAL-c174t-e64189bc114e94592efb5f7f4e8761d60e2dc3eb0bdf835ec1d30676392d7fc53</originalsourceid><addsrcrecordid>eNpFkM1KAzEURoMotlbBJ5As3UzNnWR-spSirVAplAq6GjKZGxtJJzWZQfr2tlh19cHH4SwOIdfAxsBYerdDNeay5CdkCEwWCWM5nJIhK4RMMsZfB-Qixg_GALK0PCcDLjNR5qUYktXSO6Te0EVQjr6hih21LV3i1mFr49q273S6P4PV9LnXPir6Zbv1kVRtQ2forPa10h0Gut05H-wlOTPKRbw67oi8PD6sJrNkvpg-Te7niYZCdAnmAkpZawCBUmQyRVNnpjACyyKHJmeYNppjzerGlDxDDQ1neZFzmTaF0Rkfkdsf7zb4zx5jV21s1OicatH3seKQyhQk5-If1cHHGNBU22A3KuwqYNWhYbVvWB0a7tGbo7WvN9j8gb_R-Df0jGxU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3129219334</pqid></control><display><type>article</type><title>Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Kadkhodaei, Sara ; Hatefi, Atousa ; Pedramnia, Shahrzad ; Godini, Elham ; Khalili-Samani, Saman ; Saniee, Parastoo ; Sarrafnejad, Abdolfattah ; Salmanian, Ali-Hatef ; Sotoudeh, Masoud ; Graham, David Y ; Malekzadeh, Reza ; Siavoshi, Farideh</creator><creatorcontrib>Kadkhodaei, Sara ; Hatefi, Atousa ; Pedramnia, Shahrzad ; Godini, Elham ; Khalili-Samani, Saman ; Saniee, Parastoo ; Sarrafnejad, Abdolfattah ; Salmanian, Ali-Hatef ; Sotoudeh, Masoud ; Graham, David Y ; Malekzadeh, Reza ; Siavoshi, Farideh</creatorcontrib><description>The relationship between oral and gastric yeasts and their role in the colonization of Helicobacter pylori in the stomach was studied. Four groups of 221, 7, 44, and 10 patients were used for the isolation of H. pylori and oral and gastric yeasts. In Group 1, gastric biopsies were used for the isolation of H. pylori and yeast, rapid urease test (RUT), staining with Gram's and hematoxylin &amp; eosin (H&amp;E), and immunohistochemistry (IHC) methods. In the other three groups, DNAs extracted from H. pylori and yeasts were used for the amplification of H. pylori-specific genes. Wet mounts of yeasts in Group 2 were examined to observe intracellular bacteria and released EVs. Among 221 patients, 65 (29.3%) had oral yeast, 35 (15.8%) H. pylori, and 31 (14%) gastric yeast. Culture of oral yeasts showed a significant correlation with the detection of H. pylori by IHC (10.3%), Gram stain (9%), RUT (6.3%), H&amp;E (4.9%), and culture (4%) (p &lt; 0.05). Gram-stained biopsies showed the occurrence of yeast and H. pylori, and the release of EVs from yeast. Detection of similar H. pylori genes in oral and gastric yeasts from patients in Group 2 showed their common source. Oral yeasts in Groups 3 and 4 also carried H. pylori genes. Wet mount preparations of yeasts showed intracellular bacteria inside the yeast vacuole and the release of EVs that could carry H. pylori. Oral yeast protects its intracellular H. pylori and releases it inside EVs to safely reach gastric mucosa. Yeast, as the environmental reservoir of H. pylori, plays a crucial role in bacterial reinfection after successful eradication.</description><identifier>ISSN: 0749-503X</identifier><identifier>ISSN: 1097-0061</identifier><identifier>EISSN: 1097-0061</identifier><identifier>DOI: 10.1002/yea.3983</identifier><identifier>PMID: 39548684</identifier><language>eng</language><publisher>England</publisher><ispartof>Yeast (Chichester, England), 2024-11</ispartof><rights>2024 John Wiley &amp; Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c174t-e64189bc114e94592efb5f7f4e8761d60e2dc3eb0bdf835ec1d30676392d7fc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39548684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kadkhodaei, Sara</creatorcontrib><creatorcontrib>Hatefi, Atousa</creatorcontrib><creatorcontrib>Pedramnia, Shahrzad</creatorcontrib><creatorcontrib>Godini, Elham</creatorcontrib><creatorcontrib>Khalili-Samani, Saman</creatorcontrib><creatorcontrib>Saniee, Parastoo</creatorcontrib><creatorcontrib>Sarrafnejad, Abdolfattah</creatorcontrib><creatorcontrib>Salmanian, Ali-Hatef</creatorcontrib><creatorcontrib>Sotoudeh, Masoud</creatorcontrib><creatorcontrib>Graham, David Y</creatorcontrib><creatorcontrib>Malekzadeh, Reza</creatorcontrib><creatorcontrib>Siavoshi, Farideh</creatorcontrib><title>Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori</title><title>Yeast (Chichester, England)</title><addtitle>Yeast</addtitle><description>The relationship between oral and gastric yeasts and their role in the colonization of Helicobacter pylori in the stomach was studied. Four groups of 221, 7, 44, and 10 patients were used for the isolation of H. pylori and oral and gastric yeasts. In Group 1, gastric biopsies were used for the isolation of H. pylori and yeast, rapid urease test (RUT), staining with Gram's and hematoxylin &amp; eosin (H&amp;E), and immunohistochemistry (IHC) methods. In the other three groups, DNAs extracted from H. pylori and yeasts were used for the amplification of H. pylori-specific genes. Wet mounts of yeasts in Group 2 were examined to observe intracellular bacteria and released EVs. Among 221 patients, 65 (29.3%) had oral yeast, 35 (15.8%) H. pylori, and 31 (14%) gastric yeast. Culture of oral yeasts showed a significant correlation with the detection of H. pylori by IHC (10.3%), Gram stain (9%), RUT (6.3%), H&amp;E (4.9%), and culture (4%) (p &lt; 0.05). Gram-stained biopsies showed the occurrence of yeast and H. pylori, and the release of EVs from yeast. Detection of similar H. pylori genes in oral and gastric yeasts from patients in Group 2 showed their common source. Oral yeasts in Groups 3 and 4 also carried H. pylori genes. Wet mount preparations of yeasts showed intracellular bacteria inside the yeast vacuole and the release of EVs that could carry H. pylori. Oral yeast protects its intracellular H. pylori and releases it inside EVs to safely reach gastric mucosa. Yeast, as the environmental reservoir of H. pylori, plays a crucial role in bacterial reinfection after successful eradication.</description><issn>0749-503X</issn><issn>1097-0061</issn><issn>1097-0061</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpFkM1KAzEURoMotlbBJ5As3UzNnWR-spSirVAplAq6GjKZGxtJJzWZQfr2tlh19cHH4SwOIdfAxsBYerdDNeay5CdkCEwWCWM5nJIhK4RMMsZfB-Qixg_GALK0PCcDLjNR5qUYktXSO6Te0EVQjr6hih21LV3i1mFr49q273S6P4PV9LnXPir6Zbv1kVRtQ2forPa10h0Gut05H-wlOTPKRbw67oi8PD6sJrNkvpg-Te7niYZCdAnmAkpZawCBUmQyRVNnpjACyyKHJmeYNppjzerGlDxDDQ1neZFzmTaF0Rkfkdsf7zb4zx5jV21s1OicatH3seKQyhQk5-If1cHHGNBU22A3KuwqYNWhYbVvWB0a7tGbo7WvN9j8gb_R-Df0jGxU</recordid><startdate>20241115</startdate><enddate>20241115</enddate><creator>Kadkhodaei, Sara</creator><creator>Hatefi, Atousa</creator><creator>Pedramnia, Shahrzad</creator><creator>Godini, Elham</creator><creator>Khalili-Samani, Saman</creator><creator>Saniee, Parastoo</creator><creator>Sarrafnejad, Abdolfattah</creator><creator>Salmanian, Ali-Hatef</creator><creator>Sotoudeh, Masoud</creator><creator>Graham, David Y</creator><creator>Malekzadeh, Reza</creator><creator>Siavoshi, Farideh</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20241115</creationdate><title>Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori</title><author>Kadkhodaei, Sara ; Hatefi, Atousa ; Pedramnia, Shahrzad ; Godini, Elham ; Khalili-Samani, Saman ; Saniee, Parastoo ; Sarrafnejad, Abdolfattah ; Salmanian, Ali-Hatef ; Sotoudeh, Masoud ; Graham, David Y ; Malekzadeh, Reza ; Siavoshi, Farideh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c174t-e64189bc114e94592efb5f7f4e8761d60e2dc3eb0bdf835ec1d30676392d7fc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kadkhodaei, Sara</creatorcontrib><creatorcontrib>Hatefi, Atousa</creatorcontrib><creatorcontrib>Pedramnia, Shahrzad</creatorcontrib><creatorcontrib>Godini, Elham</creatorcontrib><creatorcontrib>Khalili-Samani, Saman</creatorcontrib><creatorcontrib>Saniee, Parastoo</creatorcontrib><creatorcontrib>Sarrafnejad, Abdolfattah</creatorcontrib><creatorcontrib>Salmanian, Ali-Hatef</creatorcontrib><creatorcontrib>Sotoudeh, Masoud</creatorcontrib><creatorcontrib>Graham, David Y</creatorcontrib><creatorcontrib>Malekzadeh, Reza</creatorcontrib><creatorcontrib>Siavoshi, Farideh</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Yeast (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kadkhodaei, Sara</au><au>Hatefi, Atousa</au><au>Pedramnia, Shahrzad</au><au>Godini, Elham</au><au>Khalili-Samani, Saman</au><au>Saniee, Parastoo</au><au>Sarrafnejad, Abdolfattah</au><au>Salmanian, Ali-Hatef</au><au>Sotoudeh, Masoud</au><au>Graham, David Y</au><au>Malekzadeh, Reza</au><au>Siavoshi, Farideh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori</atitle><jtitle>Yeast (Chichester, England)</jtitle><addtitle>Yeast</addtitle><date>2024-11-15</date><risdate>2024</risdate><issn>0749-503X</issn><issn>1097-0061</issn><eissn>1097-0061</eissn><abstract>The relationship between oral and gastric yeasts and their role in the colonization of Helicobacter pylori in the stomach was studied. Four groups of 221, 7, 44, and 10 patients were used for the isolation of H. pylori and oral and gastric yeasts. In Group 1, gastric biopsies were used for the isolation of H. pylori and yeast, rapid urease test (RUT), staining with Gram's and hematoxylin &amp; eosin (H&amp;E), and immunohistochemistry (IHC) methods. In the other three groups, DNAs extracted from H. pylori and yeasts were used for the amplification of H. pylori-specific genes. Wet mounts of yeasts in Group 2 were examined to observe intracellular bacteria and released EVs. Among 221 patients, 65 (29.3%) had oral yeast, 35 (15.8%) H. pylori, and 31 (14%) gastric yeast. Culture of oral yeasts showed a significant correlation with the detection of H. pylori by IHC (10.3%), Gram stain (9%), RUT (6.3%), H&amp;E (4.9%), and culture (4%) (p &lt; 0.05). Gram-stained biopsies showed the occurrence of yeast and H. pylori, and the release of EVs from yeast. Detection of similar H. pylori genes in oral and gastric yeasts from patients in Group 2 showed their common source. Oral yeasts in Groups 3 and 4 also carried H. pylori genes. Wet mount preparations of yeasts showed intracellular bacteria inside the yeast vacuole and the release of EVs that could carry H. pylori. Oral yeast protects its intracellular H. pylori and releases it inside EVs to safely reach gastric mucosa. Yeast, as the environmental reservoir of H. pylori, plays a crucial role in bacterial reinfection after successful eradication.</abstract><cop>England</cop><pmid>39548684</pmid><doi>10.1002/yea.3983</doi></addata></record>
fulltext fulltext
identifier ISSN: 0749-503X
ispartof Yeast (Chichester, England), 2024-11
issn 0749-503X
1097-0061
1097-0061
language eng
recordid cdi_proquest_miscellaneous_3129219334
source Wiley-Blackwell Read & Publish Collection
title Role of Oral Yeast in Replenishing Gastric Mucosa with Yeast and Helicobacter pylori
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T22%3A44%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Role%20of%20Oral%20Yeast%20in%20Replenishing%20Gastric%20Mucosa%20with%20Yeast%20and%20Helicobacter%20pylori&rft.jtitle=Yeast%20(Chichester,%20England)&rft.au=Kadkhodaei,%20Sara&rft.date=2024-11-15&rft.issn=0749-503X&rft.eissn=1097-0061&rft_id=info:doi/10.1002/yea.3983&rft_dat=%3Cproquest_cross%3E3129219334%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c174t-e64189bc114e94592efb5f7f4e8761d60e2dc3eb0bdf835ec1d30676392d7fc53%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3129219334&rft_id=info:pmid/39548684&rfr_iscdi=true