The oilseed rape R2R3‐type BnaMYB78 transcription factor regulates leaf senescence by modulating PCD and chlorophyll degradation

• Published in: Physiologia plantarum 2024-11, Vol.176 (6), p.e14629-n/a
• Main Authors: Sun, Mengting, Yan, Yunru, Han, Feng, Zhao, Yuxin, Chen, Bisi, Cui, Xing, Li, Chun, Yang, Bo, Zhao, Yiting, Jiang, Yuan‐Qing
• Format: Article
• Language: English
• Subjects: Abscission; Apoptosis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - physiology; Assaying; Brassica napus; Brassica napus - genetics; Brassica napus - metabolism; Brassica napus - physiology; Cell death; Chlorophyll; Chlorophyll - metabolism; Chromatin; Degradation; Developmental stages; Disintegration; Electrophoretic mobility; Environmental degradation; Gene Expression Regulation, Plant; Genes; Immunoprecipitation; Leaves; Nutrient utilization; Oilseed crops; Oilseeds; Plant growth; Plant Leaves - genetics; Plant Leaves - metabolism; Plant Leaves - physiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Senescence - genetics; Plants, Genetically Modified; Rape plants; Rapeseed; Salicylic acid; Senescence; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0031-9317; 1399-3054; 1399-3054
• DOI: 10.1111/ppl.14629

Leaf senescence is the final stage of plant growth and development, characterized by chlorophyll degradation, organelle disintegration, and nutrient redistribution and utilization. This stage involves a complex and precise regulatory network, and the underlying mechanisms are not fully understood. Oilseed rape (Brassica napus L.) is one of the most important oil crops in China and globally. Therefore, mining and studying the key factors modulating leaf senescence and abscission in oilseed rape is of great importance to improve its yielding and nutrient use efficiency. In this study, we report that BnaMYB78 positively regulates leaf senescence in oilseed rape. As a transcriptional activator located in the nucleus, BnaMYB78 can bind to the SMRE7 (A/G)CC(T/A)AA(C/T) cis‐element in vitro and positively regulate the expression of BnaPBS3, BnaMC9, and BnaNYC1 in oilseed rape. Overexpression of BnaMYB78 leads to chlorophyll degradation and premature leaf senescence in both Arabidopsis thaliana and oilseed rape. During this process, the expression of several genes associated with salicylic acid (SA) synthesis, chlorophyll metabolism, and senescence‐associated genes (SAGs) was upregulated, including BnaPPH, BnaSAG14, BnaMC9, BnaPBS3, BnaNYC1, and BnaICS1, which facilitate the progression of programmed cell death (PCD). Further analyses demonstrated that BnaMYB78 activates the promoter activities of BnaMC9, BnaPBS3, and BnaNYC1 in a dual‐luciferase reporter assay. Electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation coupled with quantitative PCR (ChIP‐qPCR) assays revealed that BnaMYB78 directly binds to the promoter regions of these downstream target genes. In summary, our data demonstrate that BnaMYB78 modulates cell death and leaf senescence.