Genetic Interaction Between F‐Box Encoding UCC1 and RRM3 Regulates Growth Rate, Cell Size, and Stress Tolerance in Saccharomyces cerevisiae

• Published in: Journal of biochemical and molecular toxicology 2024-12, Vol.38 (12), p.e70059-n/a
• Main Authors: Pandita, Monika, Shoket, Heena, Kumar, Rakesh, Bairwa, Narendra K.
• Format: Article
• Language: English
• Subjects: Acetic acid; Acid resistance; Apoptosis; Carbon; Carbon cycle; Carbon sources; Cell morphology; Cell size; Cellular stress response; Citrate synthase; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA helicase; DNA Helicases - genetics; DNA Helicases - metabolism; Energy metabolism; Epistasis, Genetic; F-Box Proteins - genetics; F-Box Proteins - metabolism; Fungi; Genes; Genetic analysis; gene‐gene interactions; Glucose metabolism; Glyoxylate cycle; Growth rate; Hydrogen peroxide; metabolism; Morphology; Network analysis; Proteasomes; Protein turnover; RecQ Helicases; Replication; replication fork; Replication forks; Retrograde transport; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Stress, Physiological - genetics; Yeast
• ISSN: 1095-6670; 1099-0461; 1099-0461
• DOI: 10.1002/jbt.70059

ABSTRACT Ucc1, an F‐box motif‐containing protein of Saccharomyces cerevisiae encoded by UCC1 regulates energy metabolism through proteasomal degradation of citrate synthase Cit2 and inactivation of the glyoxylate cycle when glucose is present as the main carbon source in the growth medium. Rrm3, a Pif1 family DNA helicase, encoded by RRM3 regulates the movement of the replication forks during the DNA replication process. Here in this study, we present evidence of binary genetic interaction between both the genes, UCC1 and RRM3, that determine the growth rate, cell morphology, cell size, apoptosis, and stress response. The absence of both genes UCC1 and RRM3 leads to altered cell morphology, increased growth rate, utilization of alternate carbon sources, resistance to hydrogen peroxide, and susceptibility to acetic acid‐induced apoptosis. Further, the genetic interaction network analysis shows both the genes UCC1 and RRM3 interaction through the SGS1 and cross‐link among metabolic, glyoxylate, DNA replication, and retrograde signaling pathways. Genetic integration between UCC1 and RRM3 links the cross‐talk among the metabolic, DNA replication and repair, ubiquitin‐proteasome degradation, and glyoxylate pathways.