Calcium Cross-Linked Cellulose Nanofibrils: Hydrogel Design for Local and Controlled Nitric Oxide Release

• Published in: ACS applied bio materials 2024-12, Vol.7 (12), p.8377-8388
• Main Authors: Mariano, Marcos, Naseri, Narges, Nascimento, Diego Magalhães Do, Franqui, Lidiane, Seabra, Amedea Barozzi, Mathew, Aji P, Bernardes, Juliana Silva
• Format: Article
• Language: English
• Subjects: Biocompatible Materials - chemistry; Biocompatible Materials - pharmacology; Calcium - chemistry; Calcium - metabolism; Cellulose - chemistry; Cross-Linking Reagents - chemistry; Hydrogels - chemistry; Hydrogels - pharmacology; Materials Testing; Nanofibers - chemistry; Nitric Oxide - chemistry; Nitric Oxide - metabolism; Particle Size
• ISSN: 2576-6422; 2576-6422
• DOI: 10.1021/acsabm.4c01169

Nitric oxide (NO) holds promise for wound healing due to its antimicrobial properties and role in promoting vasodilation and tissue regeneration. The local delivery of NO to target cells or organs offers significant potential in numerous biomedical applications, especially when NO donors are integrated into nontoxic viscous matrices. This study presents the development of robust cellulose nanofibril (CNF) hydrogels designed to control the release of nitric oxide (NO) generated in situ from a NO-donor molecule (S-nitrosoglutathione, GSNO) obtained from the nitrosation of its precursor molecule glutathione (GSH). CNF, efficiently isolated from sugar cane bagasse, exhibited a high aspect ratio and excellent colloidal stability in water. Although depletion forces could be observed upon the addition of GSH, this effect did not significantly alter the morphology of the CNF network at low GSH concentrations (