Evaluation of the Biological Properties of the Bacteriodaceae Family Using the Caco-2 Cell Line

• Published in: Bulletin of experimental biology and medicine 2024-11, Vol.178 (1), p.54-57
• Main Authors: Podoprigora, I. V., Das, M. S., Zakharzhevskaya, N. B., Kafarskaya, L. I., Efimov, B. A.
• Format: Article
• Language: English
• Subjects: Bacteria; Bacterial Adhesion; Bacteroidaceae; Bacteroidetes; Biomedical and Life Sciences; Biomedicine; Caco-2 Cells; Cell Biology; Drug development; Drug resistance; E coli; Electric Impedance; Electrical resistivity; Humans; Immunology and Microbiology; Internal Medicine; Laboratory Medicine; Metabolites; Microbiota; Microflora; Pathology; Preventive medicine
• ISSN: 0007-4888; 1573-8221; 1573-8221
• DOI: 10.1007/s10517-024-06281-y

Disorders in the composition of the microbiota are often associated with pathological processes in the body. The use of new biotherapeutic drugs containing bacteria from normal microflora to correct these disorders may contribute to more effective treatment. The Bacteroidaceae family dominating in microflora is a candidate for inclusion in bacterial preparations. The adhesion properties of five bacterial strains from the Bacteroidaceae family were analyzed using the Caco-2 cell line and their effect on the transepithelial electrical resistance parameters (TEER) was assessed to determine the extent of their impact on the cell monolayer integrity. This approach allowed the selection of strain B. intestinalis 181, which was characterized by a moderate adhesion activity combined with a relatively low effect on the TEER parameter.