The effect of silicate on polyamine oxidase genes in Skeletonema dohrnii

• Published in: Marine environmental research 2025-02, Vol.204, p.106860, Article 106860
• Main Authors: Teng, Wei, Shang, Xiaomei, Sun, Jun
• Format: Article
• Language: English
• Subjects: Diatom; Polyamine metabolism; Spermine
• ISSN: 0141-1136; 1879-0291; 1879-0291
• DOI: 10.1016/j.marenvres.2024.106860

By using Skeletonema dohrnii as the experimental algal species, we investigated how silicate concentration impacts the polyamine metabolism of diatoms in our experiment. Three different silicate concentrations were set for cultivation, and Skeletonema dohrnii at the exponential growth phase was selected to analyze basic physiological parameters, polyamine content, and Polyamine oxidase (PAO) gene expression under varying silicate concentrations. Results showed that low silicate concentrations led to reduced growth rate and polyamine content, with down-regulation of PAO gene expression. Conversely, high silicate concentrations did not significantly increase growth rate but did increase polyamine content and up-regulate the PAO gene. These findings suggested that changes in silicate concentration impact the growth rate and polyamine content of Skeletonema dohrnii, with the PAO gene potentially playing a role in regulating the algal response to silicate concentration variations. This study provides a foundation for further exploration of the PAO gene function in Skeletonema dohrnii. •Significant differences in the response of diatoms to different silicate concentrations.•Negative correlation between polyamine content and silicate concentration.•PAO gene expression was down-regulated in low silicate concentrations and up-regulated in high silicate environments.