A rhodamine based near-infrared fluorescent probe for selective detection of Cu2+ ions and its applications in bioimaging

A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm). TM2 exhibits highly selective recognition for Cu2+ in EtOH/H2O (1 : 1, v/v) solution with a low detection limit (2.3 μM) and a wide detection range (0–50 μM). Detectio...

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Bibliographic Details
Published in:Analytical methods 2024-12, Vol.17 (1), p.178-183
Main Authors: Chang, Zhijian, Li, Shumeng, Jia-Hai Ye, Lin, Fuyan, Chen, Yuncong, Guo, Zijian, Gong, Xuedong
Format: Article
Language:English
Subjects:
Copper
Fluorescent indicators
I.R. radiation
Medical imaging
NMR
Nuclear magnetic resonance
Recognition
Rhodamine
Titration
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Summary:A novel near-infrared fluorescent probe TM2 based on a rhodamine-bearing framework was disclosed with a large Stokes shift (100 nm). TM2 exhibits highly selective recognition for Cu2+ in EtOH/H2O (1 : 1, v/v) solution with a low detection limit (2.3 μM) and a wide detection range (0–50 μM). Detection of Cu2+ is undisturbed at physiological pH levels of 5–9. This recognition mechanism is attributed to the formation of a 1 : 1 complex between TM2 and Cu2+, validated by Job's plot, 1H NMR titration, and LC-MS experiments. Moreover, the successful fluorescence imaging of Cu2+ both in vitro and in vivo was also accomplished.
ISSN:1759-9660
1759-9679
1759-9679
DOI:10.1039/d4ay01922a