Analysis of minerals and phytochemicals in the leaves of the Ocimum kilimandscharicum plant using spectroscopic methods and screening of antibacterial activity

• Published in: Natural product research 2024-11, p.1-7
• Main Authors: Sakuntala, P, Ch Pradyutha, A, Selvaraju, R
• Format: Article
• Language: English
• ISSN: 1478-6419; 1478-6427; 1478-6427
• DOI: 10.1080/14786419.2024.2432607

This study presents an innovative X-ray diffraction (XRD) application to identify minerals in the medicinal plant . Additionally, this study includes the analysis of chemical compounds and screening antibacterial activity in the leaf extract, using Gas Chromatography-Mass Spectrometry (GC-MS) and UV-visible spectroscopy. The results of X-ray diffraction showed that the leaves are a rich source of minerals such as oxides of Potassium, Magnesium, and Calcium. The GC-MS analysis revealed that the leaf extract of possesses Eugenol (27.53%), Camphor (16.91%), Thujopsene (13.53%) as major components and UV-visible studies showed the existence of Phytochemicals such as chromophores, carotenoids, and chlorophyll. The ethanol extract of leaves showed antimicrobial activity against four microorganisms. It significantly inhibited E.Coli, and surpassing the positive control streptomycin. These findings suggest the potential of using leaves of for curing infectious diseases and can be used as a mineral supplement.