Albumin and Polysorbate-80 Coated Sterile Nanosuspensions of Mebendazole for Glioblastoma Therapy

• Published in: AAPS PharmSciTech 2024-11, Vol.25 (8), p.271, Article 271
• Main Authors: Patel, Himaxi, Patel, Akanksha, Vats, Mukti, Patel, Ketan
• Format: Article
• Language: English
• Subjects: Albumins - administration & dosage; Albumins - chemistry; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; Blood-Brain Barrier - metabolism; Brain Neoplasms - drug therapy; Cell Line, Tumor; Cell Survival - drug effects; Glioblastoma - drug therapy; Humans; Mebendazole - administration & dosage; Mebendazole - chemistry; Mebendazole - pharmacology; Nanoparticles - chemistry; Particle Size; Pharmacology/Toxicology; Pharmacy; Polysorbates - chemistry; Research Article; Solubility; Suspensions
• ISSN: 1530-9932; 1530-9932
• DOI: 10.1208/s12249-024-02978-5

The scarcity of existing and novel therapies for brain cancer has significantly affected the survival rate of glioblastoma patients. Mebendazole (MBZ), an antiparasitic agent demonstrated promising activity against brain cancer. However, poor solubility, multiple polymorphs, and insufficient permeability through blood–brain barrier (BBB) restricts its therapeutic efficacy through parenteral administration. The current study aimed to develop, optimize, and characterize sterile, injectable nanosuspension of mebendazole using parenterally acceptable stabilizers. Albumin and polysorbate 80 (PS-80) coated MBZ Nanosuspension (NS) was prepared using wet media milling technique. Design of experiment (DoE) approach was used to understand effect of drug loading versus stabilizer concentration. The optimized MBZ NS showed hydrodynamic diameter of 208.36 ± 0.24 nm with a poly dispersibility index (PDI) of 0.210 ± 0.03 and zeta potential of -20.41 ± 0.36 mV. The IC 50 value of MBZ NS in U-87 MG and LN-229 cell lines were found to be 0.49 ± 0.02 μM and 0.48 ± 0.05 μM, respectively. Additionally, MBZ NS demonstrated a 2.65-fold decrease in colony-forming efficiency and a 1.16-fold reduction in migration of the bridging area compared to MBZ. In 3D spheroids of the U-87 MG glioma cell line, MBZ NS exhibited a 50% reduction in tumor growth and increased cell apoptosis compared to the control. MBZ NS formulations were sterilized by gamma irradiation and tested as per the USP sterility test. Albumin-PS 80 coated NS is rendered to be useful parenteral delivery of mebendazole for the treatment of brain cancer. Graphical Abstract