Effect of sulfate-reducing bacteria (SRB) and dissimilatory iron-reducing bacteria (DIRB) coexistence on the transport and transformation of arsenic in sediments

• Published in: Water research (Oxford) 2025-02, Vol.270, p.122834, Article 122834
• Main Authors: Sun, Yan, Wu, Zhaoyuan, Lan, Jirong, Liu, Ying, Du, Yaguang, Ye, Hengpeng, Du, Dongyun
• Format: Article
• Language: English
• Subjects: Arsenic; Dissimilatory iron reducing bacteria; Sediments; Sulfate; Sulfate-reducing bacteria
• ISSN: 0043-1354; 1879-2448; 1879-2448
• DOI: 10.1016/j.watres.2024.122834

•The release rule of arsenic in groundwater sediments was obtained.•SRB and DIRB synergistically control the leaching or inhibition of As.•Iron and sulfur content are important electron sources for microbial control of arsenic conversion.•The process of iron and sulfur metabolism promotes the conversion and leaching of arsenic.•The conversion mechanism of arsenic in groundwater has been reinterpreted. Sulfate-reducing bacteria (SRBs) and dissimilatory iron-reducing bacteria (DIRBs) are recognized as significant contributors to the occurrence of elevated arsenic (As) levels in groundwater. However, the precise effects and underlying mechanisms of their interactions on As behavior within sediments remain poorly understood. In this investigation, we compared the impacts and mechanisms of DIRBs, SRBs, and mixed bacterial consortia on the migration behavior of As and Fe/S species. Our findings revealed that during the initial phase of the reaction (0–8 days, Stage 1), the mixed bacterial consortium facilitated As release by intensifying the reduction of Fe (III) and sulfate, resulting in a maximum As concentration 1.5 times higher than that observed with either DIRBs or SRBs in isolation. Subsequently, in the intermediate phase (8–20 days, Stage 2), the mixed consortium suppressed the synthesis of sulfate reductase and the secretion of toxic substances (e.g., o-Methyltoluene) associated with steroid degradation pathways. This inhibition consequently reduced the formation of secondary Fe minerals and the fixation of As. Finally, in the latter stage (20–30 days, Stage 3), the system responded to the threat of toxic substances by secreting significant amounts of organic acids to facilitate their decomposition. However, this process also led to the re-decomposition of iron oxides, resulting in the release of As. These observations shed light on the intricate interplay between DIRBs and SRBs within bacterial consortia, elucidating their coordinated actions in inducing the migration and transformation of arsenic. [Display omitted]