Multifunctional metabolites of Streptomyces kunmingensis BS19 from bamboo rhizosphere soil

• Published in: International microbiology 2024-11
• Main Authors: Sangeetha, Murthy, Sasirekha, Sivakumar, Mahendran, Jaganathan, Sivarajan, Anbalmani, Radhakrishnan, Manikkam, Manigundan, Kaari, Dastager, Syed G, Sengottuvelu, Singaravel, Balagurunathan, Ramasamy
• Format: Article
• Language: English
• ISSN: 1618-1905; 1618-1905
• DOI: 10.1007/s10123-024-00619-2

A pigmented bioactive molecule from Streptomyces kunmingensis BS19 was isolated, characterized, and evaluated for anti-infective, antiproliferative, and wound-healing properties. The yellow-red pigment produced from the strain BS19 showed promising activity against methicillin-resistant Staphylococcus aureus (MRSA) and Mycobacterium tuberculosis. Extracellular bioactive pigment from the strain BS19 was produced by agar surface fermentation and purified through bioassay guided preparative HPLC-based purification. Based on the results of UV, FT-IR, GC-MS, H NMR, and C NMR spectral analyses, the purified pigment was identified as a chromopeptide class of molecule with phenoxazinone chromophore. Its molecular weight was determined as the chemical formula C H N O and molecular weight 1283 g/mol. It exhibited promising antimicrobial activity against Staphylococcus aureus (ATCC 1720) and antiproliferative activity against 14 types of human cancer cell lines. It showed good in vivo wound-healing activity in the rat model. The present study explored Streptomyces kunmingensis as a newly added source for the isolation of chromopeptide antibiotics for antimicrobial, wound-healing, and anticancer applications.