Cratylia mollis lectin reduces inflammatory burden induced by multidrug-resistant Staphylococcus aureus in diabetic wounds

• Published in: Histochemistry and cell biology 2025-12, Vol.163 (1), p.13
• Main Authors: dos Santos Silva, Lucas, dos Santos Castelo Branco, Simeone Júlio, Silva, Izadora Souza Soeiro, Paiva, Miria Yasmim Miranda, Vila Nova, Beatriz Gomes, de Matos Chaves Lima, Carlos Emanuel, de Oliveira, Weslley Felix, de Paiva, Felipe Eduardo Alves, Paiva, Patrícia Maria Guedes, de Souza Monteiro, Andrea, Teixeira, Claudener Souza, Cardoso, Cléver Gomes, dos Santos Correia, Maria Tereza, Nascimento da Silva, Luís Cláudio
• Format: Article
• Language: English
• Subjects: Animals; Antimicrobial agents; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cratylia mollis; Developmental Biology; Diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Diabetes Mellitus, Experimental - pathology; Immunomodulation; Inflammation; Inflammation - drug therapy; Inflammation - metabolism; Inflammation - pathology; Lectins; Male; Methicillin-Resistant Staphylococcus aureus - drug effects; Mice; Multidrug resistance; Original Paper; Plant Lectins - chemistry; Plant Lectins - pharmacology; Staphylococcal Infections - drug therapy; Staphylococcal Infections - metabolism; Staphylococcal Infections - microbiology; Staphylococcal Infections - pathology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Streptozocin; Tumor necrosis factor-α; Wound healing; Wound Healing - drug effects; Wound infection
• ISSN: 0948-6143; 1432-119X; 1432-119X
• DOI: 10.1007/s00418-024-02330-9

In diabetes, tissue repair is impaired, increasing susceptibility to Staphylococcus aureus infections, a pathogen commonly found in wounds. The emergence of S. aureus strains that are highly resistant to antimicrobial agents highlights the urgent need for alternative therapeutic options. One promising candidate is Cramoll ( Cratylia mollis seed lectin), known for its immunomodulatory, mitogenic, and healing properties. However, its efficacy in infected diabetic wounds remains unexplored. This study evaluated the effects of topical Cramoll treatment on diabetic wounds infected by S. aureus . Diabetic Swiss mice (induced by streptozotocin) were subjected to an 8-mm wound on the back and subsequently infected with a suspension of multidrug-resistant S. aureus . During the treatment period, the wounds were clinically evaluated for inflammation and the area of injury. After seven days, samples were collected from the wounds to quantify the bacterial load and histopathological and immunological analyses. Wounds infected by S. aureus exhibited more pronounced areas and severity indices, which were significantly reduced by Cramoll treatment ( p