Electroacupuncture at Zusanli (ST36) Alleviate Intestinal Ischemia-Reperfusion Injury by Regulating the Cholinergic-miRNA 124 Pathway

Intestinal ischemia-reperfusion injury (IIRI) is common in a variety of critical diseases and acute stress, and acupuncture is a promising treatment for IIRI. The aim of this study is to explore the mechanism of electroacupuncture (EA) at Zusanli (ST36) in improving IIRI from the perspective of the...

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Published in:Neurogastroenterology and motility 2024-12, p.e14971
Main Authors: Geng, YanXia, Lv, Hai, Liu, Yu, Guo, DingDing, Zheng, JingJing, Li, YingXin, Jiang, Hua, Chen, Dong
Format: Article
Language:English
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Summary:Intestinal ischemia-reperfusion injury (IIRI) is common in a variety of critical diseases and acute stress, and acupuncture is a promising treatment for IIRI. The aim of this study is to explore the mechanism of electroacupuncture (EA) at Zusanli (ST36) in improving IIRI from the perspective of the cholinergic anti-inflammatory pathway (CAP) and miRNA 124. Seven groups of mice were performed: Control group, I/R group (IIRI model), EA group (I/R + EA), miRNA mimic group (I/R + EA + miRNA 124 mimic), miRNA inhibitor group (I/R + EA + miRNA 124 inhibitor), PNU group (I/R + EA + α7nAChR agonist), and MLA group (I/R + EA+ α7nAChR antagonist). The expression of intestinal macrophages, α7nAChR, miRNA 124, and related molecules, including p-STAT3 and IL-6, as well as histological damage (Chiu's score) and mucosal permeability (serum FD4 concentration), were detected. The serum FD4 concentrations in the EA and PNU groups were significantly lower compared to the I/R group (p 
ISSN:1365-2982
1365-2982
DOI:10.1111/nmo.14971