Moldable Alginate/Hydroxyapatite Hydrogel Loaded with Metformin Enhanced Regeneration of the Rabbit Mandibular Defects

Background Moldable hydrogel-based techniques loaded with osteoinductive agents such as metformin have become a promising field for reconstructing critical-sized bone defects, particularly in those with irregular shapes. Here, we used metformin incorporated in an alginate/hydroxyapatite hydrogel to...

Full description

Saved in:
Bibliographic Details
Published in:Journal of maxillofacial and oral surgery 2024-12, Vol.23 (6), p.1391-1404
Main Authors: Nabavizadeh, Sara S, Shadi, Mehri, Khorraminejad-Shirazi, Mohammadhossein, Daneshi, Sajad, Tavanafar, Saeid, Naseri, Reyhaneh, Abbaspour, Alireza, Alaei-Jahromi, Keivan, Talaei-Khozani, Tahereh
Format: Article
Language:English
Subjects:
Biocompatibility
Biomimetics
Bone marrow
Composite materials
Defects
Dentistry
Hydrogels
Hydroxyapatite
Medicine
Medicine & Public Health
Mineralization
Oral and Maxillofacial Surgery
Original Article
Otorhinolaryngology
Plastic Surgery
Scanning electron microscopy
Surgery
Tissue engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Moldable hydrogel-based techniques loaded with osteoinductive agents such as metformin have become a promising field for reconstructing critical-sized bone defects, particularly in those with irregular shapes. Here, we used metformin incorporated in an alginate/hydroxyapatite hydrogel to accelerate the repair of the rabbit critical-sized mandibular defect. Methods Cytotoxicity and osteoinduction of the metformin-loaded alginate/hydroxyapatite hydrogel were evaluated by culturing the osteosarcoma cell line (MG63). Moreover, in vivo bone formation was assessed in a rabbit bone defect model using computed tomography and histomorphometric analysis to compare the effects of alginate/hydroxyapatite hydrogel with or without metformin. Results The data showed that the scaffolds were not cytotoxic and enhanced osteogenic characteristics of the cells, as manifested by augmented alkaline phosphatase activity and calcium deposition . In vivo studies indicated that all the treated groups exhibited more osteogenesis with a significant increase in bone-specific cell population and less residual scaffold remnant at the defect sites compared with the control group, which was significantly prominent in the group treated with alginate/hydroxyapatite/metformin. Moreover, computed tomography scan analysis also confirmed better bone filling in all the treated groups, especially in the defects treated with alginate/hydroxyapatite/metformin hydrogel. Conclusions Both In vitro and in vivo experiments revealed that locally loaded metformin with the easy size- and shape-adapted alginate/hydroxyapatite hydrogel has proper biocompatibility and osteogenesis properties. Moreover, our study highlighted the synergistic effect of metformin and hydroxyapatite on osteogenesis. Graphical Abstract
ISSN:0972-8279
0974-942X
DOI:10.1007/s12663-023-02094-0