The glycogene alterations and potential effects in esophageal squamous cell carcinoma

• Published in: Cellular and molecular life sciences : CMLS 2024-12, Vol.81 (1), p.481-481, Article 481
• Main Authors: Feng, Xuefei, Chen, Jinyan, Lian, Jianhong, Dong, Tianyue, Gao, Yingzhen, Zhang, Xiaojuan, Zhai, Yuanfang, Zou, Binbin, Guo, Yanlin, Xu, Enwei, Cui, Yongping, Zhang, Ling
• Format: Article
• Language: English
• Subjects: Affinity chromatography; Alkynes; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; caudal vein; Cell Biology; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Copy number; Esophageal cancer; Esophageal carcinoma; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma - genetics; Esophageal Squamous Cell Carcinoma - metabolism; Esophageal Squamous Cell Carcinoma - pathology; Female; fucose; Fucosyltransferases - genetics; Fucosyltransferases - metabolism; gene expression regulation; Gene Expression Regulation, Neoplastic; Genomic analysis; genomics; Glycoproteins; Glycosylation; Humans; Immunohistochemistry; Immunoprecipitation; In vivo methods and tests; lectins; Life Sciences; Male; Metastases; Metastasis; Mice; Mice, Inbred BALB C; Mice, Nude; Middle Aged; multiomics; Original Article; p53 Protein; Polysaccharides; precipitin tests; Prognosis; Proteomics; Signal Transduction; Squamous cell carcinoma
• ISSN: 1420-682X; 1420-9071; 1420-9071
• DOI: 10.1007/s00018-024-05534-3

Background Aberrant glycosylation is one of the hallmarks of cancer. The profile of glycoprotein expression caused by abnormal glycosylation has been revealed, while abnormal glycogenes that may disturb the structure of glycans have not yet been identified in esophageal squamous cell carcinoma (ESCC). Methods Genomic alterations driven by differentially expressed glycogenes in ESCC were compared with matched normal tissues by multi-omics analysis. Immunohistochemistry, MTT, colony formation, transwell assays, subcutaneous tumor formation experiments and tail vein injection were used to study the expression and the effect on the proliferation and metastasis of the differentially expressed glycogenes POFUT1 and RPN1 in ESCC. In the alkyne fucose labeling experiment, AAL lectin affinity chromatography and immunoprecipitation were used to explore the mechanism of POFUT1 in ESCC. Results The expression of the POFUT1 and RPN1 glycogenes were upregulated, as determined by genomic copy number gain and proteomics analysis. The overexpression of POFUT1 or RPN1 was associated with poor prognosis in ESCC patients and affected the proliferation and metastasis of ESCC in vivo and in vitro. The overexpression of POFUT1 increased the overall fucosylation level and activated the Notch signaling pathway, which partially mediated POFUT1 induced pro-migration in ESCC. The regulation of malignant progression of ESCC by RPN1 may be related to the TNF signaling pathway, p53 signaling pathway, etc. Conclusions Our study fills a gap in the study of abnormal glycogenes and highlights the potential role of the POFUT1/Notch axis in ESCC. Moreover, our study identifies POFUT1 and RPN1 as promising anticancer targets in ESCC.