Influence of vibration duration on the quality of boar semen doses packed in bags or tubes

• Published in: Animal reproduction science 2025-01, Vol.272, p.107658, Article 107658
• Main Authors: Bona, Gabriel A., Rech, Rodrigo D., Leal, Leonardo A., Wolf, Lucas M., Christ, Thaís S., Schulze, Martin, Bortolozzo, Fernando P., Ulguim, Rafael R., Mellagi, Ana Paula G.
• Format: Article
• Language: English
• Subjects: Agitation; Animals; Boar semen; Male; Packaging; Semen - physiology; Semen Analysis - veterinary; Semen Preservation - methods; Semen Preservation - veterinary; Semen quality; Sperm Motility; Spermatozoa - physiology; Swine - physiology; Time Factors; Transportation; Vibration
• ISSN: 0378-4320; 1873-2232; 1873-2232
• DOI: 10.1016/j.anireprosci.2024.107658

As previous data showing the negative impact of semen agitation during transport on sperm quality was obtained only in tube-type packages, this study aimed to compare two packages for boar semen dose (bag or tube) submitted to different agitation times. Ejaculates from thirty different boars were diluted in BTS extender, packed in bags or tubes in split sample, and submitted to agitation for 0, 6, or 12 h at 100 rpm at 17 ºC. Semen doses were stored for up to 168 h and evaluated for quality traits. Analyses were conducted as repeated measures, including package type, agitation time, storage time (or incubation for TRT) and their interactions. A longer agitation time resulted in reduced sperm motility, increased pH, decreased apoptotic-like changes cells percentage, reduced mitochondrial activity, increased ROS, and decreased sperm motility after TRT (P