Melatonin induces fiber switching by improvement of mitochondrial oxidative capacity and function via NRF2/RCAN/MEF2 in the vastus lateralis muscle from both sex Zücker diabetic fatty rats

• Published in: Free radical biology & medicine 2025-02, Vol.227, p.322-335
• Main Authors: Salagre, Diego, Bajit, Habiba, Fernández-Vázquez, Gumersindo, Dwairy, Mutaz, Garzón, Ingrid, Haro-López, Rocío, Agil, Ahmad
• Format: Article
• Language: English
• Subjects: Fiber switching; MEF2; Melatonin; Mitochondrial respirometry; Obese-diabetic rats; Skeletal muscle; Vastus lateralis
• ISSN: 0891-5849; 1873-4596; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2024.12.019

The positive role of melatonin in obesity control and skeletal muscle (SKM) preservation is well known. We recently showed that melatonin improves vastus lateralis muscle (VL) fiber oxidative phenotype. However, fiber type characterization, mitochondrial function, and molecular mechanisms that underlie VL fiber switching by melatonin are still undefined. Our study aims to investigate whether melatonin induces fiber switching by NRF2/RCAN/MEF2 pathway activation and mitochondrial oxidative metabolism modulation in the VL of both sex Zücker diabetic fatty (ZDF) rats. 5-Weeks-old male and female ZDF rats (N = 16) and their age-matched lean littermates (ZL) were subdivided into two subgroups: control (C) and orally treated with melatonin (M) (10 mg/kg/day) for 12 weeks. Interestingly, melatonin increased oxidative fibers amounts (Types I and IIa) counteracting the decreased levels found in the VL of obese-diabetic rats, and upregulated NRF2, calcineurin and MEF2 expression. Melatonin also restored the mitochondrial oxidative capacity increasing the respiratory control ratio (RCR) in both sex and phenotype rats through the reduction of the proton leak component of respiration (state 4). Melatonin also improved the VL mitochondrial phosphorylation coefficient and modulated the total oxygen consumption by enhancing complex I, III and IV activity, and fatty acid oxidation (FAO) in both sex obese-diabetic rats, decreasing in male and increasing in female the complex II oxygen consumption. These findings suggest that melatonin treatment induces fiber switching in SKM improving mitochondrial functionality by NRF2/RCAN/MEF2 pathway activation. [Display omitted] •Melatonin induces Skeletal Muscle (SKM) fiber switching in both sex obese-diabetic rats.•Melatonin increases the number of type I and IIa oxidative fibers and decreases the number of type IIb glycolytic ones.•Melatonin treatment recovers mitochondrial lipid β-oxidation and oxidative capacity of SKM impaired by obesity.•Melatonin ameliorates SKM mitochondrial respiratory function and complexes I-IV activities.•Melatonin activates NRF2/RCAN/Calcineurin/MEF2 pathway improving mitochondrial oxidative metabolism and SKM fiber switching.