Study of the mechanism by which Bacillus subtilis improves the soil bacterial community environment in severely saline-alkali cotton fields

• Published in: The Science of the total environment 2024-12, Vol.958, p.178000, Article 178000
• Main Authors: Xia, Hanji, Liu, Hongguang, Gong, Ping, Li, Pengfei, Xu, Qiang, Zhang, Qian, Sun, Mingyue, Meng, Qiang, Ye, Fuhai, Yin, Weizhen
• Format: Article
• Language: English
• Subjects: Bacillus subtilis; Bacterial community structure; Severely saline-alkali cotton fields; Soil bacterial environment
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.178000

Soil salinization severely damages the soil bacterial community environment. Bacillus subtilis can improve bacterial communities and enhance crop nutrient absorption. However, the mechanism by which B. subtilis improves the bacterial community environment in heavily saline-alkali-treated cotton fields is currently unclear. Therefore, this study adopted a field plot experiment and established four bacterial treatments (0, 9, 12, and 15 kg·ha−1) to investigate the environmental improvement mechanism of B. subtilis on soil bacterial communities in severely saline alkali cotton fields was studied. Compared with the CK treatment, the application of B. subtilis significantly increased the available nitrogen (25.34 %), available phosphorus (50.894 %), available potassium (86.87 %), and urease (112.961 %) contents but significantly reduced the soil pH (1.07 %) and salt content (39.73 %) and significantly increased the proline (245.116 %) and superoxide dismutase (237.46 %) contents in the leaves and significantly reduced the malondialdehyde content (47.30 %). This is mainly because B. subtilis enhances the diversity of bacterial communities and affects catalase, urease, phosphatase, and protease activities, thereby promoting nutrient release in the soil and improving soil fertility; specifically, B. subtilis promotes the secretion of oxalic acid, formic acid, malic acid, and soluble total sugars in cotton roots. The organic acids in root exudates lower the soil pH and chelate with salt ions in the soil, reducing the concentration of soluble salts and providing a suitable environment for B. subtilis. Soluble total sugars can provide energy and carbon sources for bacteria, maintaining the health and diversity of rhizosphere bacterial communities. The results of the principal component analysis revealed that the application rate of B. subtilis was 12 kg·ha−1, which had the greatest effect on improving the soil bacterial community in severely saline-alkali cotton fields. The research results provide a theoretical basis and practical reference for microbial improvement in severely saline-alkali land in arid areas. [Display omitted] •Bacillus subtilis enhances bacterial diversity.•Enhances soil-bacteria-cotton interactions.•Optimal at 12 kg·ha-1 application.