Discovery of LINC01614 associated with the SPP1 gene in colorectal cancer

• Published in: Pathology, research and practice research and practice, 2025-02, Vol.266, p.155761, Article 155761
• Main Authors: Norouzinasab, Fatemeh, Salimian, Niloufar, Mokhtari, Khatere, Akbari, Mohammadarian, Maghsoudloo, Mazaher, Entezari, Maliheh, Taheriazam, Afshin, Farahani, Najma, Hashemi, Mehrdad
• Format: Article
• Language: English
• Subjects: Colorectal cancer; LINC01614; LncRNAs; Prognostic markers; RT-qPCR; SPP1; TGF-β
• ISSN: 0344-0338; 1618-0631; 1618-0631
• DOI: 10.1016/j.prp.2024.155761

Colorectal cancer (CRC) is a prevalent malignancy worldwide, driven by complex molecular mechanisms. This study aims to elucidate the role of lncRNAs within TGF-β pathway, a crucial signaling pathway in CRC progression, focusing specifically on their interaction with the SPP1 gene. We employed a multi-faceted approach, starting with comprehensive in silico analyses to identify candidate lncRNAs potentially involved in TGF-β pathway regulation. These candidates were further validated through experimental RT-qPCR assays, comparing lncRNA expression profiles in CRC tissues to adjacent normal samples. Our findings revealed novel lncRNA candidates with significant associations with SPP1 in CRC, highlighting their potential regulatory roles in the TGF-β pathway. This integrative study underscores the importance of combining computational predictions with laboratory experimentation to uncover complex regulatory networks in cancer, providing insights into new therapeutic targets and diagnostic biomarkers for CRC.