Achatina fulica Haemocyanin-Derived Peptides as Novel Antimicrobial Agents

Haemocyanin-derived peptides were previously found in semi-purified fractions of mucus secretion from the snail Achatina fulica, which exhibited an inhibitory effect on Staphylococcus aureus strains. Here, an in silico rational design strategy was employed to generate new antimicrobial peptides (AMP...

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Bibliographic Details
Published in:Biochimie 2024-12
Main Authors: Pereira, Andrés, Suarez, Libardo, Roman, Tanya, Guzmán, Fanny, Sierra, Leidy, Rincón-Orozco, Bladimiro, Hidalgo, William
Format: Article
Language:English
Subjects:
antibacterial activity
Antimicrobial peptides
cytotoxic activity
Escherichia coli
Haemocyanin
Staphylococcus aureus
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Summary:Haemocyanin-derived peptides were previously found in semi-purified fractions of mucus secretion from the snail Achatina fulica, which exhibited an inhibitory effect on Staphylococcus aureus strains. Here, an in silico rational design strategy was employed to generate new antimicrobial peptides (AMPs) from A. fulica haemocyanin-derived peptides (AfH). The designed peptides were chemically synthetized using the Fmoc strategy, and their antimicrobial activity against Escherichia coli and S. aureus strains was evaluated using the broth microdilution method. In addition, the cytotoxic activity on Vero, HaCat, and human erythrocyte cells was also determined. The results demonstrated that 15-residue alpha-helical and cationic synthetic peptides exhibited the highest biological activity against Gram-positive strains, with minimum inhibitory concentrations (MIC) in the range from 7.5 to 30 μM. The positive selectivity index suggests a higher selectivity, primarily on the microorganisms evaluated, but not on eukaryotic cells. In this study, A. fulica hemocyanin was identified as an appropriate protein model for the rational design of AMPs against bacteria of public health significance. Further studies are required to evaluate the activity of the peptides on Gram-negative bacteria other than E. coli. [Display omitted] •Novel peptides from Achatina fulica haemocyanin were designed and synthesized.•Peptides exhibited strong antimicrobial activity against Staphylococcus aureus.•Peptides showed selectivity for bacterial cells over eukaryotic cells.•Minimum inhibitory concentrations ranged from 7.5 to 30 μM for S. aureus.•Further research needed to evaluate effectiveness against Gram-negative bacteria
ISSN:0300-9084
1638-6183
1638-6183
DOI:10.1016/j.biochi.2024.12.007