Lipidomics of Caco-2 Cells Under Simulated Microgravity Conditions

Microgravity may profoundly impact the cardiovascular system, skeletal muscle system, and immune system of astronauts. At the cellular level, microgravity may also affect cell proliferation, differentiation, and growth, as well as lipid metabolism. In this work, we investigated lipid changes in Caco...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-12, Vol.25 (23), p.12638
Main Authors: Tolle, Giulia, Serreli, Gabriele, Deiana, Monica, Moi, Loredana, Zavattari, Patrizia, Pantaleo, Antonella, Manis, Cristina, El Faqir, Mohammed Amine, Caboni, Pierluigi
Format: Article
Language:English
Subjects:
Apoptosis
Astronauts
Caco-2 Cells
Cell cycle
Cell differentiation
Ceramides
Ceramides - metabolism
Dextran
Dextran Sulfate
Disease
Endoplasmic reticulum
Humans
Inflammation
Inflammatory bowel disease
Lipid Metabolism
Lipidomics - methods
Lipids
Lipopolysaccharides - pharmacology
Metabolism
Metabolites
Multivariate analysis
Muscles
Permeability
Small intestine
Sphingomyelins - metabolism
Sulfates
Weightlessness
Weightlessness Simulation
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Summary:Microgravity may profoundly impact the cardiovascular system, skeletal muscle system, and immune system of astronauts. At the cellular level, microgravity may also affect cell proliferation, differentiation, and growth, as well as lipid metabolism. In this work, we investigated lipid changes in Caco-2 cells cultured in a clinostat for 24 h under simulated microgravity conditions (SMC). Complex lipids were measured using a UHPLC-QTOF/MS platform, and the data were subjected to multivariate analysis. Under SMC, levels of ceramides Cer 18:0;O2/16:0, Cer 18:1;O2/16:0, Cer 18:1; O2/22:0, Cer 18:1;O2/24:0, and Cer 18:2;O2/24:0 were found to be upregulated, while sphingomyelins SM 16:1;O2/16:0, SM 16:1;O2/18:1, SM 18:1;O2/24:0, and SM 18:2;O2/24:0 were found to be downregulated. On the other hand, considering that sphingolipids are involved in the process of inflammation, we also treated Caco-2 cells with dextran sodium sulfate (DSS) to induce cell inflammation and lipopolysaccharide (LPS) to induce cell immune responses. As a result, we observed similar lipid dysregulation, indicating that SMC may exert a condition similar to inflammation. Our lipidomics strategy provides new insights into the altered metabolic pathway of ceramides and sphingomyelins of Caco-2 cells under SMC.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms252312638