Molecular characterization of the E2 conjugating enzyme LinfUbc13 in Leishmania infantum

• Published in: Archives of biochemistry and biophysics 2025-02, Vol.764, p.110272, Article 110272
• Main Authors: Rodrigues da Silva, Eduardo Vagner, Torres, Caroline, Nemamiah Escolarique Ribeiro, Hariel, Santana Travaglini Berti de Correia, Camila Rolemberg, de Oliveira de Castro, Taissa, da Costa Mancin, Giovanna, Zanchetta Venancio, Mayla Gabriela, Abdel Baqui, Munira Muhammad, Teixeira, Felipe Roberti, Gomes, Marcelo Damário
• Format: Article
• Language: English
• Subjects: E2-UB; Kinetoplastid; Leishmania; Proteasome; UBC13; Ubiquitin
• ISSN: 0003-9861; 1096-0384; 1096-0384
• DOI: 10.1016/j.abb.2024.110272

UBC13 is an orthologue of Homo sapiens ubiquitin-conjugation E2 enzymes described in Leishmania mexicana, a null mutant lacking this gene cannot be produced, suggesting essential functions in this parasite. Leishmania infantum is an etiological agent of visceral leishmaniasis, the most severe type of disease that is potentially fatal if untreated. The ubiquitination process has been targeted for leishmanicidal compounds, indicating its essential function in parasite homeostasis. Therefore, the molecular characterization of the ubiquitination process may provide a better understanding of the molecular and cellular basis of leishmaniasis. Here, we characterized the gene LINF_350017900 in Leishmania infantum, which was named LinfUBC13, an E2 orthologue of UBC13 in Leishmania mexicana and the UBE2D family in Homo sapiens, sharing 72–74 % identity with UBE2D1, UBE2D2, and UBE2D3. LinfUbc13 contains conserved catalytic residues, including Cys86 and the HPN motif, which are essential for ubiquitin-conjugating activity. Structural analysis revealed a high similarity between LinfUbc13 and human UBE2D proteins, with a root-mean-square deviation (RMSD) of 0.4 Å, suggesting conserved functions. Recombinant LinfUbc13 was expressed and shown to accept ubiquitin from E1, forming a thioester intermediate. Functional assays demonstrated that LinfUbc13 transfers ubiquitin to p53 through human HDM2 E3 ligase, confirming its role in ubiquitination. Subcellular localization showed that LinfUbc13 was distributed throughout the parasite cytoplasm. These findings highlight the conserved nature of the ubiquitin-proteasome system between Leishmania infantum and Homo sapiens, showing that LinfUbc13 is an E2 enzyme that plays a crucial role in parasitic development. [Display omitted] •LinfUBC13, an E2 orthologue of UBC13 in Leishmania mexicana and the UBE2D family in Homo sapiens, sharing 72–74 % identity with UBE2D1, UBE2D2, and UBE2D3.•Recombinant LinfUbc13 is able to accept ubiquitin from E1, forming a thioester intermediate.•LinfUbc13 is present in the cytosol of parasites.•LinfUbc13 is an essential gene.