Detecting gene expression in Caenorhabditis elegans

• Published in: Genetics (Austin) 2025-01, Vol.229 (1), p.1-108
• Main Authors: Calarco, John A, Taylor, Seth R, Miller, 3rd, David M
• Format: Article
• Language: English
• Subjects: Animals; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Gene Expression Profiling - methods; Proteomics - methods; Sequence Analysis, RNA - methods; Single-Cell Analysis - methods
• ISSN: 1943-2631; 1943-2631
• DOI: 10.1093/genetics/iyae167

Reliable methods for detecting and analyzing gene expression are necessary tools for understanding development and investigating biological responses to genetic and environmental perturbation. With its fully sequenced genome, invariant cell lineage, transparent body, wiring diagram, detailed anatomy, and wide array of genetic tools, Caenorhabditis elegans is an exceptionally useful model organism for linking gene expression to cellular phenotypes. The development of new techniques in recent years has greatly expanded our ability to detect gene expression at high resolution. Here, we provide an overview of gene expression methods for C. elegans, including techniques for detecting transcripts and proteins in situ, bulk RNA sequencing of whole worms and specific tissues and cells, single-cell RNA sequencing, and high-throughput proteomics. We discuss important considerations for choosing among these techniques and provide an overview of publicly available online resources for gene expression data.