Deep proteomics and network pharmacology reveal sex- and age-shared neuropathic pain signatures in mouse dorsal root ganglia

• Published in: Pharmacological research 2025-01, Vol.211, p.107552, Article 107552
• Main Authors: Grundtner, Sabrina, Sondermann, Julia R., Xian, Feng, Malzl, Daniel, Segelcke, Daniel, Pogatzki-Zahn, Esther M., Menche, Jörg, Gómez-Varela, David, Schmidt, Manuela
• Format: Article
• Language: English
• Subjects: Dorsal root ganglia; MEFISTO; Neuropathic pain; Pediatric; Proteomics; Systems pharmacology
• ISSN: 1043-6618; 1096-1186; 1096-1186
• DOI: 10.1016/j.phrs.2024.107552

Our understanding of how sex and age influence chronic pain at the molecular level is still limited with wide-reaching consequences for adolescent patients. Here, we leveraged deep proteome profiling of mouse dorsal root ganglia (DRG) from adolescent (4-week-old) and adult (12-week-old) male and female mice to investigate the establishment of neuropathic pain in the spared nerve injury (SNI)-model in parallel. We quantified over 12,000 proteins, including notable ion channels involved in pain, highlighting the sensitivity of our approach. Differential expression revealed sex- and age-dependent proteome changes upon nerve injury. In contrast to most previous studies, our comprehensive dataset enabled us to determine differentially expressed proteins (DEPs), which were shared between male and female mice of both age groups. Among these, the vast majority (94 %) were also expressed and, in part, altered in human DRG of neuropathic pain patients, indicating evolutionary conservation. Proteome signatures represented numerous targets of FDA-approved drugs comprising both (i) known pain therapeutics (e.g. Pregabalin and opioids) and, importantly, (ii) compounds with high potential for future re-purposing, e.g. Ptprc-modulators and Epoetins. Protein network and multidimensional analysis uncovered distinct hubs of sex- and age-shared biological pathways impacted by neuropathic pain, such as neuronal activity and synaptic function, DNA-damage, and neuroimmune interactions. Taken together, our results capture the complexity of nerve injury-associated DRG alterations in mice at the network level, moving beyond single-candidate studies. Consequently, we provide an innovative resource of the molecular landscape of neuropathic pain, enabling novel opportunities for translational pain research and network-based drug discovery. [Display omitted] •Proteome-dataset of nerve injury-associated signatures in mouse DRG.•Sex and adolescent age as key biological variables for neuropathic pain development.•High translational value of identified sex- and age-shared proteome signatures.•Matching with human data and systems pharmacology reveals potential drug targets.•Innovative resource for translational pain research and network-based drug discovery.