Degradation of myosmine by a novel bacterial strain Sphingopyxis sp. J-6 and its degradation pathways

• Published in: Journal of hazardous materials 2024-12, Vol.486, p.136996, Article 136996
• Main Authors: Dang, Bingjun, Gao, Hui, Jia, Wei, Zhang, Yuwei, Xu, Zicheng, Han, Dan, Yang, Jinchu, Huang, Yao, Chen, Zheng, Wang, Yadi, Duan, Yingqiu, Yuan, Ruohua, Qiao, Yimeng, Yu, Hexiang, Jin, Pengfei, Ai, Hangting, Huang, Wuxing
• Format: Article
• Language: English
• Subjects: Degradation; Myosmine; Pathway; Sphingopyxis sp; Tobacco alkaloids
• ISSN: 0304-3894; 1873-3336; 1873-3336
• DOI: 10.1016/j.jhazmat.2024.136996

This study isolated a myosmine-degrading bacterial strain J-6 from tobacco-growing soil. The identification of this strain revealed it to be a new species within the genus Sphingopyxis. Analysis of the myosmine degradation products by HPLC, preparative HPLC, and UHPLC-MS/MS identified 8 metabolites, among which 3-pyridylacetic acid (3-PAA), 5-(3-pyridyl)tetrahydrofuranone-2 (PTHF), and 4-hydroxy-4-(3-pyridyl)butanoic acid (HPBA) were three novel metabolites that were not previously found in microbial degradation of tobacco alkaloids. Interestingly, these metabolites have been observed in the nicotine metabolic pathways of humans and animals. In addition, 3-PAA, which is believed to be the major end product of nicotine metabolism in humans, is also found to be an end product of myosmine degradation in strain J-6. Based on the identified metabolites and genomic analysis, a previously unreported bacterial degradation pathway for tobacco alkaloids was proposed. The downstream part of this pathway for converting SP to 3-PAA resembles the pathway for mammalian metabolism of SP to 3-PAA. Overall, the findings in this study offer novel insights into the degradation pathways and mechanisms of myosmine, which will deepen our understanding on the fate of myosmine both in the environment and within the human body. [Display omitted] •A novel myosmine-degrading bacterial strain Sphingopyxis sp. J-6 was isolated.•The metabolite 3-PAA is found to be an end product of myosmine degradation.•Genomic analysis revealed putative VPP pathway gene cluster.•A previously unreported bacterial degradation pathway for myosmine was proposed.