Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors

Celosia argentea is a plant known for producing bioactive compounds, including betalains, which possess various biological and pharmaceutical properties. This study aimed to investigate the effect of biotic and abiotic elicitors on betalains production and their antioxidant activity in cell suspensi...

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Bibliographic Details
Published in:Scientific reports 2025-01, Vol.15 (1), p.376
Main Authors: Mueangnak, Kanchanok, Kitwetcharoen, Haruthairat, Thanonkeo, Sudarat, Klanrit, Preekamol, Apiraksakorn, Jirawan, Klanrit, Poramaporn, Klanrit, Poramate, Thanonkeo, Pornthap
Format: Article
Language:English
Subjects:
631/449
631/61
Acetates - metabolism
Acetates - pharmacology
Antioxidants - metabolism
Betalains - metabolism
Cell Culture Techniques - methods
Cells, Cultured
Celosia - metabolism
Chitosan - pharmacology
Cyclopentanes - metabolism
Cyclopentanes - pharmacology
Humanities and Social Sciences
multidisciplinary
Oxylipins - metabolism
Oxylipins - pharmacology
Salicylic Acid - metabolism
Salicylic Acid - pharmacology
Science
Science (multidisciplinary)
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Summary:Celosia argentea is a plant known for producing bioactive compounds, including betalains, which possess various biological and pharmaceutical properties. This study aimed to investigate the effect of biotic and abiotic elicitors on betalains production and their antioxidant activity in cell suspension cultures of C. argentea . Various concentrations of chitosan, yeast extract, salicylic acid, methyl jasmonate, copper sulfate (CuSO 4 ), and cobalt chloride (CoCl 2 ) were evaluated. The results revealed that chitosan, salicylic acid, methyl jasmonate, and CuSO 4 significantly improved betalains production in the cell suspension cultures. Among these elicitors, chitosan at 5.0 mg/L and CuSO 4 at 6.4 µM were the most effective in enhancing betalains production, yielding the highest concentrations of 4.65 and 4.99 mg/g dry weight, respectively. Notably, the betalains derived from the elicitor-treated cultures exhibited greater antioxidant activity compared to the control. These findings suggest that chitosan and CuSO 4 are promising elicitors for sustainable in vitro production of betalains from C. argentea cell suspension cultures on a commercial scale, owing to their ability to enhance betalains production and antioxidant activity.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-83096-x