Micropropagation Protocols of New Prunus Hybrids with Significant Rootstock Potential in Fruit Breeding and Cultivation

• Published in: Erwerbsobstbau 2023-10, Vol.65 (5), p.1359-1364
• Main Authors: Bulunuz Palaz, Esra, Ugur, Remzi, Yaman, Mehmet
• Format: Article
• Language: English
• Subjects: Agricultural research; Agriculture; Biomedical and Life Sciences; Breeding methods; Cross-breeding; Cultivation; Fruit cultivation; Fruits; Hybrids; Life Sciences; Mass production; Micropropagation; Original Article; Originalbeitrag; Plant breeding; Plant Sciences; Production methods; Prunus dulcis; Research centers; Rooting; Rootstocks; Shoots
• ISSN: 0014-0309; 1439-0302
• DOI: 10.1007/s10341-023-00908-6

In vitro micropropagation is the most valid production method used for disease-free, clonal, and mass production of plants developed using unique breeding methods. This study was carried out to establish appropriate protocols for the micropropagation of 10 ( Prunus amygdalus ) × ( P. amygdalus  ×  P. persica ) hybrid rootstocks obtained by crossbreeding. The maximum micro shoot and shoot length values were obtained from the medium (MED 11) as 3.92 shoots/explant and 14.59 mm, respectively. The best rooting values were obtained from MS medium supplemented with 1.75 mgL −1 IBA + 0.1 mgL −1 BAP (MED-5) in the rooting study. The highest average root number, root length, and root diameter of shoots 2.79 pcs/explant, 14.36 mm, and 1.15 pcs/explant, respectively, were observed. It is suggested that MS media supplemented with MED-11 (0.1 mg.L −1 IBA + 1.75 mg.L −1 BAP + 0.2 mg.L −1 GA 3 ), MED-12 (0.1 mg.L −1 IBA + 2.0 mg.L −1 BAP + 0.2 mg.L −1 GA 3 ) for regeneration, MED‑5 (1.75 mg.L −1 IBA + 0.1 mg.L −1 BAP), and MED‑6 (2.0 mg.L −1 IBA + 0.1 mg.L −1 BAP) hormone combinations for rooting would be appropriate for the micropropagation of hybrid rootstocks. It is thought that the results of the study can be a guide for new studies to be carried out especially in these species.