Tissue transcriptome profiling and pathway analyses revealed novel potential biomarkers in the tumor progression of canine oral melanoma

Canine oral melanoma (COM) is an aggressive oral malignancy in dogs, mostly with metastasis. However, the understanding of total gene expression of oral melanoma (OM) at different clinical stages has been limited. The objective of this study was to identify novel mRNA biomarkers of early-stage OM (E...

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Bibliographic Details
Published in:Research in veterinary science 2023-12, Vol.165, p.105036-105036, Article 105036
Main Authors: Pisamai, Sirinun, Edwards, Steven W., Cheng, Chew Weng, Chaivichit, Phannita, Sooksiri, Mokhapoom, Yanakam, Sujittra, Maneewong, Sattabongkoch, Suriyaphol, Gunnaporn
Format: Article
Language:English
Subjects:
biomarkers
Cancer biomarkers
Canine oral melanoma
dogs
Dopachrome tautomerase
drug therapy
Forkhead box M1
gene expression
genes
melanocytes
melanoma
metastasis
neoplasm progression
reverse transcriptase polymerase chain reaction
RNA-Seq
Transcriptome
veterinary medicine
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Summary:Canine oral melanoma (COM) is an aggressive oral malignancy in dogs, mostly with metastasis. However, the understanding of total gene expression of oral melanoma (OM) at different clinical stages has been limited. The objective of this study was to identify novel mRNA biomarkers of early-stage OM (EOM) and late-stage OM (LOM). Transcriptome sequencing of 3 EOM, 5 LOM and 4 normal gingival tissues (controls) was performed. Selected transcriptome results were validated by quantitative reverse transcription-PCR (qRT-PCR) using 12 LOM and 10 controls. We found 534 differentially expressed in EOM compared with controls, whereas 696 genes in LOM were differentially expressed compared with controls (P 
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2023.105036