Metabolomics analysis of the metabolic effects of citric acid on Issatchenkia terricola WJL-G4

• Published in: Journal of bioscience and bioengineering 2023-12, Vol.136 (6), p.452-461
• Main Authors: Mei, Jiajia, Liu, Xiaojie, Liu, Xinyi, Bao, Yihong, Luo, Ting, Wang, Jinling
• Format: Article
• Language: English
• Subjects: Acid degradation; acid treatment; carbon; Citric Acid; Citric Acid Cycle; fruit juices; gluconeogenesis; glucose; glycolysis; Issatchenkia terricola WJL-G4; liquid chromatography; mass spectrometry; metabolomics; Metabolomics - methods; Metabolomics analysis; nicotinamide; phospholipids; Pichia; Pichia terricola; purines; Tricarboxylic acid cycle; wines; yeasts
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2023.09.006

In current research, yeast species Issatchenkia terricola WJL-G4 was shown to be capable of degrading citric acid, especially in the processing of fruit juice and wine. I. terricola WJL-G4 was able to use citric acid as a carbon source, but the metabolic effects of citric acid on yeast remained unclear. In this study, the metabolic effects of citric acid on I. terricola WJL-G4 were studied using liquid chromatography-mass spectrometry metabolomics technology, with glucose treatment as the control. Results showed that organic acid contents related to the extracellular tricarboxylic acid cycle (TCA) varied greatly. The metabolomics results indicated that I. terricola WJL-G4 might metabolize citric acid through the TCA pathway, and the glycolysis pathway might be inhibited; however, gluconeogenesis proceeded normally during citric acid treatment. Some fatty acids and phospholipids, along with the metabolic pathways of amino acids, vitamins, purines and nicotinamide in I. terricola WJL-G4 were also affected by the citric acid treatment. This work provided a theoretical basis for further study of the mechanism of yeast metabolism of citric acid. [Display omitted] •Citric acid culture led to extracellular TCA related organic acid changes of Issatchenkia terricola WJL-G4.•I. terricola WJL-G4 probably metabolized citric acid by TCA.•Glycolysis in I. terricola WJL-G4 might be inhibited, yet gluconeogenesis proceed normally under citric acid treatment.•The metabolic pathways of fatty acid, amino acid, vitamin, purine and nicotinamide were affected by citric acid treatment.